WB (Western Blot)
(Detection of mouse Nanog by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3, CT26, F9, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Nanog antibody AAA210927 (lot AAA210927-4) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
Rabbit anti-Mouse Nanog Polyclonal Antibody | anti-Nanog antibody
Rabbit anti-Nanog Antibody, Affinity Purified
Gene Names
Nanog; ENK; ecat4; 2410002E02Rik
Reactivity
Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
Nanog, Antibody; Rabbit anti-Nanog Antibody, Affinity Purified; ecat4; homeobox transcription factor Nanog; homeobox protein NANOG; ENK; early embryo specific expression NK-type homeobox protein; early embryo specific expression NK family; 2410002E02Rik; ES cell-associated protein 4; Homeobox protein NANOG; Nanog homeobox; anti-Nanog antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-Nanog antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 1 and 50
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of mouse Nanog by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3, CT26, F9, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Nanog antibody AAA210927 (lot AAA210927-4) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
WB (Western Blot)
(Detection of mouse Nanog by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3, CT26, F9, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Nanog antibody AAA210927 (lot AAA210927-4) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of mouse Nanog by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from F9 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Nanog antibody (AAA210927 lot 4) used for IP at 6 ug per reaction. Nanog was also immunoprecipitated by a previous lot of this antibody AAA210927 lot 3) and a second antibody against a different epitope of Nanog . For blotting immunoprecipitated Nanog, AAA210927 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)
IP (Immunoprecipitation)
(Detection of mouse Nanog by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from F9 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Nanog antibody (AAA210927 lot 4) used for IP at 6 ug per reaction. Nanog was also immunoprecipitated by a previous lot of this antibody AAA210927 lot 3) and a second antibody against a different epitope of Nanog . For blotting immunoprecipitated Nanog, AAA210927 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)
Related Product Information for anti-Nanog antibody
Nanog is a homeobox transcription factor involved in promoting embryonic stem (ES) cell self-renewal and the delay of lineage commitment.
Product Categories/Family for anti-Nanog antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
31,859 Da
NCBI Official Full Name
homeobox protein NANOG isoform 1
NCBI Official Synonym Full Names
Nanog homeobox
NCBI Official Symbol
Nanog
NCBI Official Synonym Symbols
ENK; ecat4; 2410002E02Rik
NCBI Protein Information
homeobox protein NANOG
UniProt Protein Name
Homeobox protein NANOG
UniProt Gene Name
Nanog
UniProt Synonym Gene Names
Ecat4; Enk
UniProt Entry Name
NANOG_MOUSE
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Product Notes
The Nanog nanog (Catalog #AAA210927) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Nanog Antibody, Affinity Purified reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Nanog can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the Nanog nanog for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nanog, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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