FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of MCF-7 cells using anti-NARFL/CIAO3 antibody (AAA128219).Overlay histogram showing MCF-7 cells stained with AAA128219 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-NARFL/CIAO3 Antibody (AAA128219, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
Rabbit anti-Human NARFL/CIAO3 Polyclonal Antibody | anti-NARFL/CIAO3 antibody
Anti-NARFL/CIAO3 Antibody Picoband
Gene Names
NARFL; PRN; HPRN; IOP1; LET1L
Reactivity
Human
Applications
ELISA, Flow Cytometry, Functional Assay, Western Blot
Purity
Immunogen affinity purified.
Synonyms
NARFL/CIAO3, Antibody; Anti-NARFL/CIAO3 Antibody Picoband; Cytosolic iron-sulfur assembly component 3; cytosolic iron-sulfur assembly component 3; CIAO3; NARFL; PRN; Cytosolic Fe-S cluster assembly factor NARFL; Iron-only hydrogenase-like protein 1; IOP1; Nuclear prelamin A recognition factor-like protein; Protein related to Narf; anti-NARFL/CIAO3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Applicable Applications for anti-NARFL/CIAO3 antibody
ELISA, FCM/FACS (Flow Cytometry), WB (Western Blot)
Immunogen
E coli-derived human NARFL/CIAO3 recombinant protein (Position: Y196-W476). Human NARFL/CIAO3 shares 88.3% and 86.8% amino acid (aa) sequence identity with mouse and rat NARFL/CIAO3, respectively.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Cross Reactivity
No cross-reactivity with other proteins
Preparation and Storage
At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of MCF-7 cells using anti-NARFL/CIAO3 antibody (AAA128219).Overlay histogram showing MCF-7 cells stained with AAA128219 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-NARFL/CIAO3 Antibody (AAA128219, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of MCF-7 cells using anti-NARFL/CIAO3 antibody (AAA128219).Overlay histogram showing MCF-7 cells stained with AAA128219 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-NARFL/CIAO3 Antibody (AAA128219, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
WB (Western Blot)
(Figure 1. Western blot analysis of NARFL/CIAO3 using anti-NARFL/CIAO3 antibody (AAA128219).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Jurkat whole cell lysates,Lane 2: human MCF-7 whole cell lysates,Lane 3: human 293T whole cell lysates,Lane 4: human A549 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NARFL/CIAO3 antigen affinity purified polyclonal antibody (AAA128219) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NARFL/CIAO3 at approximately 53 kDa. The expected band size for NARFL/CIAO3 is at 53 kDa.)
WB (Western Blot)
(Figure 1. Western blot analysis of NARFL/CIAO3 using anti-NARFL/CIAO3 antibody (AAA128219).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Jurkat whole cell lysates,Lane 2: human MCF-7 whole cell lysates,Lane 3: human 293T whole cell lysates,Lane 4: human A549 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NARFL/CIAO3 antigen affinity purified polyclonal antibody (AAA128219) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NARFL/CIAO3 at approximately 53 kDa. The expected band size for NARFL/CIAO3 is at 53 kDa.)
Related Product Information for anti-NARFL/CIAO3 antibody
The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Product Categories/Family for anti-NARFL/CIAO3 antibody
References
1. Hartz, P. A. Personal Communication. Baltimore, Md. 8/19/2011.2. Huang, J., Song, D., Flores, A., Zhao, Q., Mooney, S. M., Shaw, L. M., Lee, F. S. IOP1, a novel hydrogenase-like protein that modulates hypoxia-inducible factor-1-alpha activity. Biochem. J. 401: 341-352, 2007.3. Song, D., Lee, F. S. Mouse knock-out of IOP1 protein reveals its essential role in mammalian cytosolic iron-sulfur protein biogenesis. J. Biol. Chem. 286: 15797-15805, 2011.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
53,020 Da
NCBI Official Full Name
cytosolic Fe-S cluster assembly factor NARFL
NCBI Official Synonym Full Names
nuclear prelamin A recognition factor-like
NCBI Official Symbol
NARFL
NCBI Official Synonym Symbols
PRN; HPRN; IOP1; LET1L
NCBI Protein Information
cytosolic Fe-S cluster assembly factor NARFL; LET1 like/JFP15; protein related to Narf; iron-only hydrogenase-like protein 1; nuclear prelamin A recognition factor-like protein
UniProt Protein Name
Cytosolic Fe-S cluster assembly factor NARFL
UniProt Gene Name
NARFL
UniProt Synonym Gene Names
PRN; IOP1
UniProt Entry Name
NARFL_HUMAN
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Product Notes
The NARFL/CIAO3 narfl (Catalog #AAA128219) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-NARFL/CIAO3 Antibody Picoband reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NARFL/CIAO3 can be used in a range of immunoassay formats including, but not limited to, ELISA, FCM/FACS (Flow Cytometry), WB (Western Blot). Researchers should empirically determine the suitability of the NARFL/CIAO3 narfl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NARFL/CIAO3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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