WB (Western Blot)
(All lanes : Anti-NAT10 Antibody (N-Term) at 1:2000 dilutionLane 1: Human lung lysateLane 2: Hela whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 116 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit NAT10 Polyclonal Antibody | anti-NAT10 antibody
NAT10 Antibody (N-Term)
Gene Names
NAT10; ALP; Kre33; NET43
Reactivity
HumanPredicted: Mouse
Applications
ELISA, Western Blot, Flow Cytometry, Functional Assay
Purity
Purified through a protein A column, followed by peptide affinity purification.
Synonyms
NAT10, Antibody; NAT10 Antibody (N-Term); Purified Rabbit Polyclonal Antibody (Pab); N-acetyltransferase 10; 2.3.1.-; NAT10; ALP; KIAA1709; anti-NAT10 antibody
Host
Rabbit
Reactivity
Human
Predicted: Mouse
Predicted: Mouse
Clonality
Polyclonal
Isotype
Ig
Specificity
This NAT10 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 146-178 amino acids from the mouse region of mouse NAT10.
Purity/Purification
Purified through a protein A column, followed by peptide affinity purification.
Form/Format
Supplied in PBS with 0.09% (W/V) sodium azide.
Sequence Length
953
Applicable Applications for anti-NAT10 antibody
ELISA, WB (Western Blot), FCM/FACS (Flow Cytometry)
Antigen Type
Synthesized Peptide
Antigen Source
Human
Cellular Location
Nucleus, nucleolus {ECO:0000255|HAMAP- Rule:MF_03211, ECO:0000269|PubMed:12429849, ECO:0000269|PubMed:14592445, ECO:0000269|PubMed:19303003, ECO:0000269|PubMed:25653167}. Note=Nucleolar in interphase and redistributes to the perichromosomal layer and to the midbody during telophase.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks.
For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(All lanes : Anti-NAT10 Antibody (N-Term) at 1:2000 dilutionLane 1: Human lung lysateLane 2: Hela whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 116 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-NAT10 Antibody (N-Term) at 1:2000 dilutionLane 1: Human lung lysateLane 2: Hela whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 116 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
FCM/FACS (Flow Cytometry)
(Overlay histogram showing Hela cells stained with AP22316a(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22316a, 1:25 dilution) for 60 min at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was rabbit IgG1 (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
FCM/FACS (Flow Cytometry)
(Overlay histogram showing Hela cells stained with AP22316a(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22316a, 1:25 dilution) for 60 min at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was rabbit IgG1 (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
Related Product Information for anti-NAT10 antibody
Description: RNA cytidine acetyltransferase with specificity toward both 18S rRNA and tRNAs (PubMed:25653167). Catalyzes the formation of N(4)-acetylcytidine (ac4C) at positions 1337 and 1842 in 18S rRNA (By similarity). Required for early nucleolar cleavages of precursor rRNA at sites A0, A1 and A2 during 18S rRNA synthesis (PubMed:25653167). Catalyzes the formation of ac4C in serine and leucine tRNAs (By similarity). Requires the tRNA-binding adapter protein THUMBD1 for full tRNA acetyltransferase activity but not for 18S rRNA acetylation (PubMed:25653167). Can acetylate both histones and microtubules. Histone acetylation may regulate transcription and mitotic chromosome de-condensation. Activates telomerase activity by stimulating the transcription of TERT, and may also regulate telomerase function by affecting the balance of telomerase subunit assembly, disassembly, and localization. Acetylates alpha-tubulin, which may affect microtubule stability and cell division (PubMed:14592445, PubMed:17631499, PubMed:18082603, PubMed:19303003).
Bio Background: Has protein acetyltransferase activity in vitro. Can acetylate both histones and microtubules. Histone acetylation may regulate transcription and mitotic chromosome de-condensation. Activates telomerase activity by stimulating the transcription of TERT, and may also regulate telomerase function by affecting the balance of telomerase subunit assembly, disassembly, and localization. Acetylates alpha-tubulin, which may affect microtubule stability and cell division.
Bio Background: Has protein acetyltransferase activity in vitro. Can acetylate both histones and microtubules. Histone acetylation may regulate transcription and mitotic chromosome de-condensation. Activates telomerase activity by stimulating the transcription of TERT, and may also regulate telomerase function by affecting the balance of telomerase subunit assembly, disassembly, and localization. Acetylates alpha-tubulin, which may affect microtubule stability and cell division.
Product Categories/Family for anti-NAT10 antibody
References
Nagase T.,et al.DNA Res. 7:347-355(2000). Wiemann S.,et al.Genome Res. 11:422-435(2001). Ota T.,et al.Nat. Genet. 36:40-45(2004). Taylor T.D.,et al.Nature 440:497-500(2006). Lv J.,et al.Biochem. Biophys. Res. Commun. 311:506-513(2003).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
115730
NCBI Official Full Name
RNA cytidine acetyltransferase isoform b
NCBI Official Synonym Full Names
N-acetyltransferase 10
NCBI Official Symbol
NAT10
NCBI Official Synonym Symbols
ALP; Kre33; NET43
NCBI Protein Information
RNA cytidine acetyltransferase
UniProt Protein Name
RNA cytidine acetyltransferase
UniProt Gene Name
NAT10
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Product Notes
The NAT10 nat10 (Catalog #AAA290896) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NAT10 Antibody (N-Term) reacts with Human Predicted: Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NAT10 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the NAT10 nat10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NAT10, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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