IF (Immunofluorescence)
(Immunofluorescence analysis of U20S cell using NCF4 antibody. Blue: DAPI for nuclear staining.)
Rabbit NCF4 Polyclonal Antibody | anti-NCF4 antibody
NCF4 antibody
Gene Names
NCF4; NCF; P40PHOX; SH3PXD4
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Western Blot
Purity
Antibodies were purified by affinity purification using immunogen.
Synonyms
NCF4, Antibody; NCF4 antibody; MGC3810; NCF; P40PHOX; SH3PXD4; anti-NCF4 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
The antibody detects endogenous level of total NCF4 antibody.
Purity/Purification
Antibodies were purified by affinity purification using immunogen.
Form/Format
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
339
Applicable Applications for anti-NCF4 antibody
IF (Immunofluorescence), WB (Western Blot)
Immunogen Type
Recombinant Protein
Immunogen Description
Recombinant protein of human NCF4.
Target Name
NCF4
Preparation and Storage
Store at -20 degree C
IF (Immunofluorescence)
(Immunofluorescence analysis of U20S cell using NCF4 antibody. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of U20S cell using NCF4 antibody. Blue: DAPI for nuclear staining.)
WB (Western Blot)
(Western blot analysis of extracts of K562 cell lines, using NCF4 antibody.)
WB (Western Blot)
(Western blot analysis of extracts of K562 cell lines, using NCF4 antibody.)
Related Product Information for anti-NCF4 antibody
The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
Product Categories/Family for anti-NCF4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
39,017 Da
NCBI Official Full Name
neutrophil cytosol factor 4 isoform 1
NCBI Official Synonym Full Names
neutrophil cytosolic factor 4, 40kDa
NCBI Official Symbol
NCF4
NCBI Official Synonym Symbols
NCF; P40PHOX; SH3PXD4
NCBI Protein Information
neutrophil cytosol factor 4
UniProt Protein Name
Neutrophil cytosol factor 4
UniProt Gene Name
NCF4
UniProt Synonym Gene Names
SH3PXD4; NCF-4; p40phox
UniProt Entry Name
NCF4_HUMAN
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Product Notes
The NCF4 ncf4 (Catalog #AAA301090) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NCF4 antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NCF4 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the NCF4 ncf4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NCF4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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