WB (Western Blot)
(Western blot analysis of extracts of THP1 cells, using Phospho-p40phox (Thr154) Antibody.)
Rabbit anti-Human, Mouse p40phox Polyclonal Antibody | anti-NCF4 antibody
Phospho-p40phox (Thr154) Antibody
Gene Names
NCF4; NCF; CGD3; P40PHOX; SH3PXD4
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Peptide affinity purification
Synonyms
p40phox, Antibody; Phospho-p40phox (Thr154) Antibody; CGD3; MGC3810; NCF 4; NCF; NCF-4; Ncf4; NCF4_HUMAN; Neutrophil cytosol factor 4; Neutrophil cytosolic factor 4; Neutrophil NADPH oxidase factor 4; p40-phox; p40phox; SH3 and PX domain-containing protein 4; SH3PXD4; anti-NCF4 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Phospho-p40 phox (Thr154) Antibody detects endogenous levels of p40 phox only when phosphorylated at Thr154.
Purity/Purification
Peptide affinity purification
Form/Format
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
339
Applicable Applications for anti-NCF4 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human p40phox around the phosphorylation site of Thr154.
Conjugation
Unconjugated
Epitope
Phospho Thr154
Important
For western blot, incubate membrane with diluted primary Ab in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of extracts of THP1 cells, using Phospho-p40phox (Thr154) Antibody.)
WB (Western Blot)
(Western blot analysis of extracts of THP1 cells, using Phospho-p40phox (Thr154) Antibody.)
IHC (Immunohiostchemistry)
(AAA321360 at 1/100 staining Human thyroid cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohiostchemistry)
(AAA321360 at 1/100 staining Human thyroid cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot)
(Western blot analysis of extracts from THP1 cells, using Phospho-p40phox (Thr154) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with nonphospho-blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from THP1 cells, using Phospho-p40phox (Thr154) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with nonphospho-blocking peptide.)
Related Product Information for anti-NCF4 antibody
Function: Component of the NADPH-oxidase, a multicomponent enzyme system responsible for the oxidative burst in which electrons are transported from NADPH to molecular oxygen, generating reactive oxidant intermediates. It may be important for the assembly and/or activation of the NADPH-oxidase complex.
Subunit Structure: Component of an NADPH oxidase complex composed of a heterodimer formed by the membrane proteins CYBA and CYBB and the cytosolic subunits NCF1, NCF2 and NCF4. NCF4 interacts primarily with NCF2 to form a complex with NCF1.
Similarity: The PB1 domain mediates the association with NCF2/p67-PHOX.The PX domain mediates interaction with membranes enriched in phosphatidylnositol 3-phosphate.
Subunit Structure: Component of an NADPH oxidase complex composed of a heterodimer formed by the membrane proteins CYBA and CYBB and the cytosolic subunits NCF1, NCF2 and NCF4. NCF4 interacts primarily with NCF2 to form a complex with NCF1.
Similarity: The PB1 domain mediates the association with NCF2/p67-PHOX.The PX domain mediates interaction with membranes enriched in phosphatidylnositol 3-phosphate.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
40kDa
NCBI Official Full Name
neutrophil cytosol factor 4 isoform 1
NCBI Official Synonym Full Names
neutrophil cytosolic factor 4
NCBI Official Symbol
NCF4
NCBI Official Synonym Symbols
NCF; CGD3; P40PHOX; SH3PXD4
NCBI Protein Information
neutrophil cytosol factor 4
UniProt Protein Name
Neutrophil cytosol factor 4
UniProt Gene Name
NCF4
UniProt Synonym Gene Names
SH3PXD4; NCF-4; p40phox
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Product Notes
The NCF4 ncf4 (Catalog #AAA321360) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-p40phox (Thr154) Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's p40phox can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the NCF4 ncf4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p40phox, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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