IHC (Immunohistochemistry)
(Figure 7: Immunohistochemistry Validation of NCOA4 in Rat lungImmunohistochemical analysis of paraffin-embedded rat tissue using anti-NCOA4 antibody (AAA11046) at 1ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 degree C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)
Rabbit NCOA4 Polyclonal Antibody | anti-NCOA4 antibody
NCOA4 (IN) Antibody
IHC (Immunohistochemistry)
(Figure 7: Immunohistochemistry Validation of NCOA4 in Rat lungImmunohistochemical analysis of paraffin-embedded rat tissue using anti-NCOA4 antibody (AAA11046) at 1ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 degree C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)
IHC (Immunohistochemistry)
(Figure 7: Immunohistochemistry Validation of NCOA4 in Rat lungImmunohistochemical analysis of paraffin-embedded rat tissue using anti-NCOA4 antibody (AAA11046) at 1ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 degree C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)
IHC (Immunohistchemistry)
(Figure 6: Immunohistochemistry Validation of NCOA4 in Mouse lungImmunohistochemical analysis of paraffin-embedded mouse tissue using anti-NCOA4 antibody (AAA11046) at 1ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 degree C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)
IHC (Immunohistchemistry)
(Figure 6: Immunohistochemistry Validation of NCOA4 in Mouse lungImmunohistochemical analysis of paraffin-embedded mouse tissue using anti-NCOA4 antibody (AAA11046) at 1ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 degree C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)
IHC (Immunohistochemistry)
(Figure 5: Immunohistochemistry Validation of NCOA4 in Human lungImmunohistochemical analysis of paraffin-embedded human mouse tissue using anti-NCOA4 antibody (AAA11046) at 1ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 degree C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)
IHC (Immunohistochemistry)
(Figure 5: Immunohistochemistry Validation of NCOA4 in Human lungImmunohistochemical analysis of paraffin-embedded human mouse tissue using anti-NCOA4 antibody (AAA11046) at 1ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 degree C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)
IF (Immunofluorescence)
(Figure 4: Immunofluorescence Validation of NCOA4 in Daudi CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed Daudi cells labeling NCOA4 with AAA11046 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
IF (Immunofluorescence)
(Figure 4: Immunofluorescence Validation of NCOA4 in Daudi CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed Daudi cells labeling NCOA4 with AAA11046 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
WB (Western Blot)
(Figure 3: Western Blot Validation in Rat TissuesLoading: 15ug of lysates per lane.Antibodies: NCOA4 AAA11046, 1ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.)
WB (Western Blot)
(Figure 3: Western Blot Validation in Rat TissuesLoading: 15ug of lysates per lane.Antibodies: NCOA4 AAA11046, 1ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.)
WB (Western Blot)
(Figure 2: Western Blot Validation in Mouse Tissues Loading: 15ug of lysates per lane. Antibodies: NCOA4 AAA11046, 1ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.)
WB (Western Blot)
(Figure 2: Western Blot Validation in Mouse Tissues Loading: 15ug of lysates per lane. Antibodies: NCOA4 AAA11046, 1ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.)
WB (Western Blot)
(Figure 1: Western Blot Validation in Human Cell LinesLoading: 15ug of lysates per lane. Antibodies: NCOA4 AAA11046, 0.5ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.)
WB (Western Blot)
(Figure 1: Western Blot Validation in Human Cell LinesLoading: 15ug of lysates per lane. Antibodies: NCOA4 AAA11046, 0.5ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.)
Zhang et al. Oncogene. 2021; 40(8): 1425-1439
Guo et al. J Periodontal Res. 2021; 56(3): 523-534
Mou et al. BMC Cancer. 2021; 21(1):18
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Product Notes
The NCOA4 ncoa4 (Catalog #AAA11046) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NCOA4 (IN) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NCOA4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB), Immunofluorescence (IF). WB: 0.5-1ug/mL; IHC: 1ug/mL; IF: 20ug/mL. Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence in human samples and Immunohistochemistry in human, mouse and rat samples. All other applications and species not yet tested. Researchers should empirically determine the suitability of the NCOA4 ncoa4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NCOA4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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