IHC (Immunohiostchemistry)
(AAA322867 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample wasthen blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody)
Rabbit NDE1 Polyclonal Antibody | anti-NDE1 antibody
NDE1 Antibody
Gene Names
NDE1; NDE; LIS4; MHAC; NUDE; NUDE1; HOM-TES-87
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Peptide affinity purification
Synonyms
NDE1, Antibody; NDE1 Antibody; FLJ20101; HOM TES 87; LIS1 interacting protein NUDE1 rat homolog; LIS1 interacting protein NUDE1; LIS4; NDE 1; NDE1; NDE1_HUMAN; Nuclear distribution gene E homolog 1; Nuclear distribution protein nudE homolog 1; NUDE 1; NudE; NudE nuclear distribution gene E homolog 1 (A. nidulans); NudE nuclear distribution gene E homolog 1; NUDE1; anti-NDE1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
NDE1 antibody detects endogenous levels of NDE1.
Purity/Purification
Peptide affinity purification
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
335
Applicable Applications for anti-NDE1 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human NDE1, corresponding to a region within N-terminal amino acids.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IHC (Immunohiostchemistry)
(AAA322867 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample wasthen blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody)
IHC (Immunohiostchemistry)
(AAA322867 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample wasthen blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody)
WB (Western Blot)
(Western blot analysis of extracts from various samples, using NDE1 antibody. Lane 1: 293 treated with blocking peptide. Lane 2: 293; Lane 3: mouse brain;)
WB (Western Blot)
(Western blot analysis of extracts from various samples, using NDE1 antibody. Lane 1: 293 treated with blocking peptide. Lane 2: 293; Lane 3: mouse brain;)
Related Product Information for anti-NDE1 antibody
Function: Required for centrosome duplication and formation and function of the mitotic spindle. Essential for the development of the cerebral cortex. May regulate the production of neurons by controlling the orientation of the mitotic spindle during division of cortical neuronal progenitors of the proliferative ventricular zone of the brain. Orientation of the division plane perpendicular to the layers of the cortex gives rise to two proliferative neuronal progenitors whereas parallel orientation of the division plane yields one proliferative neuronal progenitor and a post-mitotic neuron. A premature shift towards a neuronal fate within the progenitor population may result in an overall reduction in the final number of neurons and an increase in the number of neurons in the deeper layers of the cortex.
Subunit Structure: Self-associates. Interacts with CNTRL, LIS1, dynein, SLMAP and TCP1 (By similarity). Interacts with CENPF, dynactin, tubulin gamma, PAFAH1B1, PCM1 and PCNT. Interacts with ZNF365.
Post-translational Modifications: Phosphorylated in mitosis. Phosphorylated in vitro by CDC2. Phosphorylation at Thr-246 is essential for the G2/M transition (By similarity).
Similarity: Belongs to the nudE family.
Subunit Structure: Self-associates. Interacts with CNTRL, LIS1, dynein, SLMAP and TCP1 (By similarity). Interacts with CENPF, dynactin, tubulin gamma, PAFAH1B1, PCM1 and PCNT. Interacts with ZNF365.
Post-translational Modifications: Phosphorylated in mitosis. Phosphorylated in vitro by CDC2. Phosphorylation at Thr-246 is essential for the G2/M transition (By similarity).
Similarity: Belongs to the nudE family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
40 kDa
NCBI Official Full Name
nuclear distribution protein nudE homolog 1
NCBI Official Synonym Full Names
nudE neurodevelopment protein 1
NCBI Official Symbol
NDE1
NCBI Official Synonym Symbols
NDE; LIS4; MHAC; NUDE; NUDE1; HOM-TES-87
NCBI Protein Information
nuclear distribution protein nudE homolog 1
UniProt Protein Name
Nuclear distribution protein nudE homolog 1
UniProt Gene Name
NDE1
UniProt Synonym Gene Names
NUDE; NudE
Similar Products
Product Notes
The NDE1 nde1 (Catalog #AAA322867) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NDE1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NDE1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the NDE1 nde1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NDE1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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