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IF (Immunofluorescence)
(Immunofluorescent analysis of 293 cells using AAA229068 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Rabbit anti-Human NDFIP1 Polyclonal Antibody | anti-NDFIP1 antibody
NDFIP1 Antibody
Gene Names
NDFIP1; N4WBP5
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry, Western Blot, ELISA
Purity
Affinity Purified
Synonyms
NDFIP1, Antibody; NDFIP1 Antibody; NEDD4 family-interacting protein 1; NDFIP1; N4WBP5; PSEC0192; PSEC0223; Breast cancer-associated protein SGA-1M; NEDD4 WW domain-binding protein 5; Putative MAPK-activating protein PM13; Putative NF-kappa-B-activating protein 164; Putative NFKB and MAPK-activating protein; anti-NDFIP1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
Liquid
Sequence Length
221
Applicable Applications for anti-NDFIP1 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA
Immunogen
Recombinant human NEDD4 family-interacting protein 1 protein
Conjugation
Non-conjugated
Storage Buffer
Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-69514 / sc-69517 / sc-69516
Preparation and Storage
Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.
IF (Immunofluorescence)
(Immunofluorescent analysis of 293 cells using AAA229068 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
IF (Immunofluorescence)
(Immunofluorescent analysis of 293 cells using AAA229068 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
IHC (Immunohistochemisry)
(Immunohistochemistry of paraffin-embedded human adrenal gland tissue using AAA229068 at dilution of 1:100)
IHC (Immunohistochemisry)
(Immunohistochemistry of paraffin-embedded human adrenal gland tissue using AAA229068 at dilution of 1:100)
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human breast cancer using AAA229068 at dilution of 1:100)
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human breast cancer using AAA229068 at dilution of 1:100)
WB (Western Blot)
(Western blotAll lanes: NDFIP1 antibody at 8ug/mlLane 1: 293T whole cell lysateLane 2: Hela whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 25,17 kDaObserved band size: 25 kDa)
WB (Western Blot)
(Western blotAll lanes: NDFIP1 antibody at 8ug/mlLane 1: 293T whole cell lysateLane 2: Hela whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 25,17 kDaObserved band size: 25 kDa)
Related Product Information for anti-NDFIP1 antibody
Activates HECT domain-containing E3 ubiquitin-protein ligases, including NEDD4 and ITCH, and consequently modulates the stability of their targets. As a result, controls many cellular processes. Prevents chronic T-helper cells-mediated inflammation by activating ITCH and thus controlling JUNB degradation (By similarity). In cortical neurons, mediates the ubiquitination of SLC11A2/DMT1 by NEDD4L, leading to down-regulation of the divalent metal transporter and protection of the cells from cobalt and iron toxicity (PubMed:19706893). Modulates EGFR signaling through multiple pathways. In particular, may regulate the ratio of AKT1-to-MAPK8 signaling in response to EGF, acting on AKT1 probably through PTEN destabilization and on MAPK8 through ITCH-dependent MAP2K4 inactivation. As a result, may control cell growth rate (PubMed:20534535). Enhances the ubiquitination of BRAT1 mediated by E3 ubiquitin-protein ligases: NEDD4, NEDD4L and ITCH, and is required for the nuclear localization of ubiquitinated BRAT1 (PubMed:25631046).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
16,915 Da
NCBI Official Full Name
NEDD4 family-interacting protein 1
NCBI Official Synonym Full Names
Nedd4 family interacting protein 1
NCBI Official Symbol
NDFIP1
NCBI Official Synonym Symbols
N4WBP5
NCBI Protein Information
NEDD4 family-interacting protein 1
UniProt Protein Name
NEDD4 family-interacting protein 1
UniProt Gene Name
NDFIP1
UniProt Synonym Gene Names
N4WBP5
UniProt Entry Name
NFIP1_HUMAN
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Product Notes
The NDFIP1 ndfip1 (Catalog #AAA229068) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NDFIP1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NDFIP1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the NDFIP1 ndfip1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NDFIP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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