IHC (Immunohistochemisry)
(Anti-NDUFA1 antibody, AAA45064, IHC(F)IHC(F): Rat Cardiac Muscle Tissue)
Rabbit NDUFA1 Polyclonal Antibody | anti-NDUFA1 antibody
Anti-NDUFA1 antibody
Gene Names
NDUFA1; MWFE; ZNF183; CI-MWFE
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunohistochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Synonyms
NDUFA1, Antibody; Anti-NDUFA1 antibody; NADH dehydrogenase[ubiquinone] 1 alpha subcomplex subunit 1; NADH dehydrogenase(ubiquinone) 1 alpha subcomplex, 1, 7.5kDa; CI MWFE antibody; CI-MWFE antibody; Complex I MWFE antibody; complex I MWFE subunit antibody; Complex I-MWFE antibody; MWFE antibody; MWFE, B. taurus, homolog of antibody; NADH dehydrogenase(ubiquinone) 1 alpha subcomplex 1 7.5kDa antibody; NADH dehydrogenase(ubiquinone) 1 alpha subcomplex 1 antibody; NADH dehydrogenase[ubiquinone] 1 alpha subcomplex subunit 1 antibody; NADH oxidoreductase subunit MWFE antibody; NADH ubiquinone oxidoreductase(complex 1) antibody; NADH ubiquinone oxidoreductase MWFE subunit antibody; NADH-ubiquinone oxidoreductase 1 alpha subcomplex, 1 antibody; NADH-ubiquinone oxidoreductase MWFE subunit antibody; NDUA1_HUMAN antibody; NDUFA 1 antibody; NDUFA1 antibody; Type I dehydrogenase antibody; ZNF 183 antibody; ZNF183 antibody; anti-NDUFA1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
70
Applicable Applications for anti-NDUFA1 antibody
ICC (Immunocytochemistry), IHC (Immunohistochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human NDUFA1 (54-70aa ISGVDRYYVSKGLENID), different from the related rat and mouse sequences by one amino acid.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
IHC (Immunohistochemisry)
(Anti-NDUFA1 antibody, AAA45064, IHC(F)IHC(F): Rat Cardiac Muscle Tissue)
IHC (Immunohistochemisry)
(Anti-NDUFA1 antibody, AAA45064, IHC(F)IHC(F): Rat Cardiac Muscle Tissue)
IHC (Immunohiostchemistry)
(Anti-NDUFA1 antibody, AAA45064, IHC(P)IHC(P): Rat Cardiac Muscle Tissue)
IHC (Immunohiostchemistry)
(Anti-NDUFA1 antibody, AAA45064, IHC(P)IHC(P): Rat Cardiac Muscle Tissue)
WB (Western Blot)
(Anti-NDUFA1 antibody, AAA45064, Western blottingLane 1: Rat Cardiac Muscle Tissue LysateLane 2: Rat Liver Tissue LysateLane 3: Rat Kidney Tissue LysateLane 4: Rat Brain Tissue LysateLane 5: HT1080 Cell Lysate)
WB (Western Blot)
(Anti-NDUFA1 antibody, AAA45064, Western blottingLane 1: Rat Cardiac Muscle Tissue LysateLane 2: Rat Liver Tissue LysateLane 3: Rat Kidney Tissue LysateLane 4: Rat Brain Tissue LysateLane 5: HT1080 Cell Lysate)
Related Product Information for anti-NDUFA1 antibody
Description: Rabbit IgG polyclonal antibody for NADH dehydrogenase[ubiquinone] 1 alpha subcomplex subunit 1(NDUFA1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human, Mouse, Rat.
Background: NDUFA1(NADH-UBIQUINONE OXIDOREDUCTASE 1 ALPHA SUBCOMPLEX, 1), also called MWFE, B. TAURUS, HOMOLOG OF, encodes a subunit of mitochondrial NADH: ubiquinone oxidoreductase, also known as mitochondrial complex I. The NDUFA1 gene is mapped to chromosome Xq24. The deduced polypeptide sequence of NDUFA1 was found to have an N-terminal hydrophobic domain, likely to be a transmembrane domain, and a C-terminal hydrophilic domain. And the NDUFA1 gene contains 3 exons and spans about 5.0 kb of genomic DNA. Complementation with hamster Ndufa1 cDNA restored the rotenone-sensitive complex I activity of these mutant cells to approximately 100% of the parent cell activity. The findings established that the MWFE polypeptide is absolutely essential for an active complex I in mammals. The NDUFA1 peptide is one of about 31 components of the "hydrophobic protein"(HP) fraction of complex I which is involved in proton translocation. Thus the NDUFA1 peptide may also participate in that function.
Background: NDUFA1(NADH-UBIQUINONE OXIDOREDUCTASE 1 ALPHA SUBCOMPLEX, 1), also called MWFE, B. TAURUS, HOMOLOG OF, encodes a subunit of mitochondrial NADH: ubiquinone oxidoreductase, also known as mitochondrial complex I. The NDUFA1 gene is mapped to chromosome Xq24. The deduced polypeptide sequence of NDUFA1 was found to have an N-terminal hydrophobic domain, likely to be a transmembrane domain, and a C-terminal hydrophilic domain. And the NDUFA1 gene contains 3 exons and spans about 5.0 kb of genomic DNA. Complementation with hamster Ndufa1 cDNA restored the rotenone-sensitive complex I activity of these mutant cells to approximately 100% of the parent cell activity. The findings established that the MWFE polypeptide is absolutely essential for an active complex I in mammals. The NDUFA1 peptide is one of about 31 components of the "hydrophobic protein"(HP) fraction of complex I which is involved in proton translocation. Thus the NDUFA1 peptide may also participate in that function.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
8,072 Da
NCBI Official Full Name
NADH dehydrogenase
NCBI Official Synonym Full Names
NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 1, 7.5kDa
NCBI Official Symbol
NDUFA1
NCBI Official Synonym Symbols
MWFE; ZNF183; CI-MWFE
NCBI Protein Information
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1; complex I-MWFE; type I dehydrogenase; complex I MWFE subunit; NADH oxidoreductase subunit MWFE; NADH:ubiquinone oxidoreductase (complex 1); NADH-ubiquinone oxidoreductase MWFE subunit
UniProt Protein Name
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1
UniProt Gene Name
NDUFA1
UniProt Synonym Gene Names
CI-MWFE
UniProt Entry Name
NDUA1_HUMAN
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Product Notes
The NDUFA1 ndufa1 (Catalog #AAA45064) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-NDUFA1 antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NDUFA1 can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IHC (Immunohistochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the NDUFA1 ndufa1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NDUFA1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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