WB (Western Blot)
(Detection of human and mouse NDUFA5 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, and mouse TCMK-1, cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NDUFA5 antibody AAA213274 (lot AAA213274-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human, Mouse NDUFA5 Polyclonal Antibody | anti-NDUFA5 antibody
Rabbit anti-NDUFA5 Antibody, Affinity Purified
Gene Names
NDUFA5; B13; NUFM; UQOR13; CI-13kB; CI-13KD-B
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
NDUFA5, Antibody; Rabbit anti-NDUFA5 Antibody, Affinity Purified; Complex I-13kD-B; UQOR13; ubiquinone reductase; type I dehydrogenase; NUFM; NADH-ubiquinone oxidoreductase 13 kDa-B subunit; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5, 13kDa; complex I subunit B13; complex I 13kDa subunit B; CI-13KD-B; CI-13kB; B13; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5; NADH:ubiquinone oxidoreductase subunit A5; anti-NDUFA5 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-NDUFA5 antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Orangutan, Gorilla, Chimpanzee
Immunogen
Between 66 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse NDUFA5 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, and mouse TCMK-1, cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NDUFA5 antibody AAA213274 (lot AAA213274-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human and mouse NDUFA5 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, and mouse TCMK-1, cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NDUFA5 antibody AAA213274 (lot AAA213274-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human NDUFA5 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T and cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NDUFA5 antibody AAA213274 (lot AAA213274-1) used for IP at 6 ug per reaction. NDUFA5 was also immunoprecipitated by rabbit anti-NDUFA5 antibody BL21734. For blotting immunoprecipitated NDUFA5, AAA213274 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
IP (Immunoprecipitation)
(Detection of human NDUFA5 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T and cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NDUFA5 antibody AAA213274 (lot AAA213274-1) used for IP at 6 ug per reaction. NDUFA5 was also immunoprecipitated by rabbit anti-NDUFA5 antibody BL21734. For blotting immunoprecipitated NDUFA5, AAA213274 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
Related Product Information for anti-NDUFA5 antibody
NDUFA5 is a conserved protein that comprises the B13 subunit of complex I of the mitochondrial respiratory chain. NDUFA5 localizes to the inner mitochondrial membrane, where it is thought to aid in the transfer of electrons from NADH to ubiquinone [taken from NCBI Entrez Gene (Gene ID: 4698)].
Product Categories/Family for anti-NDUFA5 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
NADH dehydrogenase
NCBI Official Synonym Full Names
NADH:ubiquinone oxidoreductase subunit A5
NCBI Official Symbol
NDUFA5
NCBI Official Synonym Symbols
B13; NUFM; UQOR13; CI-13kB; CI-13KD-B
NCBI Protein Information
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5
UniProt Protein Name
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5
UniProt Gene Name
NDUFA5
UniProt Synonym Gene Names
CI-13kD-B
UniProt Entry Name
NDUA5_HUMAN
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Product Notes
The NDUFA5 ndufa5 (Catalog #AAA213274) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-NDUFA5 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NDUFA5 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the NDUFA5 ndufa5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NDUFA5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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