WB (Western Blot)
(Detection of human NET1 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NET1 antibody AAA212121 (lot AAA212121-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human NET1 Polyclonal Antibody | anti-NET1 antibody
Rabbit anti-NET1 Antibody, Affinity Purified
Gene Names
NET1; NET1A; ARHGEF8
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
NET1, Antibody; Rabbit anti-NET1 Antibody, Affinity Purified; p65 Net1 proto-oncogene protein; ARHGEF8; small GTP-binding protein regulator; Rho guanine nucleotide exchange factor 8; Rho guanine nucleotide exchange factor (GEF) 8; Proto-oncogene p65 Net1; neuroepithelioma transforming gene 1; neuroepithelial cell-transforming gene 1 protein; NET1A; epididymis secretory sperm binding protein; guanine nucleotide regulatory protein (oncogene); Neuroepithelial cell-transforming gene 1 protein; neuroepithelial cell transforming 1; anti-NET1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-NET1 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 546 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human NET1 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NET1 antibody AAA212121 (lot AAA212121-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human NET1 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NET1 antibody AAA212121 (lot AAA212121-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human NET1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NET1 antibody AAA212121 (lot AAA212121-2) used for IP at 6 ug per reaction. For blotting immunoprecipitated NET1, AAA212121 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human NET1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NET1 antibody AAA212121 (lot AAA212121-2) used for IP at 6 ug per reaction. For blotting immunoprecipitated NET1, AAA212121 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-NET1 antibody
Neuroepithelial cell-transforming gene 1 protein (NET1) functions as a guanine exchange factor (GEF) for RhoA and plays a role in regulating apoptosis and the response to DNA damage.
Product Categories/Family for anti-NET1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
61,885 Da
NCBI Official Full Name
neuroepithelial cell-transforming gene 1 protein isoform 1
NCBI Official Synonym Full Names
neuroepithelial cell transforming 1
NCBI Official Symbol
NET1
NCBI Official Synonym Symbols
NET1A; ARHGEF8
NCBI Protein Information
neuroepithelial cell-transforming gene 1 protein
UniProt Protein Name
Neuroepithelial cell-transforming gene 1 protein
UniProt Gene Name
NET1
UniProt Synonym Gene Names
ARHGEF8
UniProt Entry Name
ARHG8_HUMAN
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Product Notes
The NET1 net1 (Catalog #AAA212121) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-NET1 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NET1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the NET1 net1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NET1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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