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product-image-AAA292050_WB10.jpg WB (Western Blot) (Sample: BV2 Cell (Mouse) Lysate at 40 ugPrimary: Anti- Netrin G1 ligand (bs-6710R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 70 kDObserved band size: 76 kD)

Rabbit Netrin G1 ligand Polyclonal Antibody | anti-LRRC4C antibody

Netrin G1 ligand Polyclonal Antibody

Gene Names
LRRC4C; NGL1; NGL-1
Reactivity
Rat, Dog, Cow
Applications
Immunofluorescence, Immunohistochemistry, Immunohistochemistry, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Netrin G1 ligand, Antibody; Netrin G1 ligand Polyclonal Antibody; Leucine-rich repeat-containing protein 4C; Netrin-G1 ligand; NGL-1; LRRC4C; KIAA1580; NGL1; anti-LRRC4C antibody
Ordering
Host
Rabbit
Reactivity
Rat, Dog, Cow
Clonality
Polyclonal
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Applicable Applications for anti-LRRC4C antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(Sample: BV2 Cell (Mouse) Lysate at 40 ugPrimary: Anti- Netrin G1 ligand (bs-6710R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 70 kDObserved band size: 76 kD)

product-image-AAA292050_WB10.jpg WB (Western Blot) (Sample: BV2 Cell (Mouse) Lysate at 40 ugPrimary: Anti- Netrin G1 ligand (bs-6710R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 70 kDObserved band size: 76 kD)

WB (Western Blot)

(Dilution: Sample: Hela Cell (Human) Lysate at 40 ugPrimary: Anti- Netrin G1 ligand (bs-6710R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 70 kDObserved band size: 76 kD)

product-image-AAA292050_WB11.jpg WB (Western Blot) (Dilution: Sample: Hela Cell (Human) Lysate at 40 ugPrimary: Anti- Netrin G1 ligand (bs-6710R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 70 kDObserved band size: 76 kD)

IF (Immunofluorescence)

(Tissue/cell: mouse brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37 degree C for 20 min;Incubation: Anti-NGL1 Polyclonal Antibody, Unconjugated(bs-6710R) 1:200, overnight at 4 degree C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37 degree C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei)

product-image-AAA292050_IF13.jpg IF (Immunofluorescence) (Tissue/cell: mouse brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37 degree C for 20 min;Incubation: Anti-NGL1 Polyclonal Antibody, Unconjugated(bs-6710R) 1:200, overnight at 4 degree C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37 degree C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei)

IF (Immunofluorescence)

(Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37 degree C for 20 min;Incubation: Anti-NGL1 Polyclonal Antibody, Unconjugated(bs-6710R) 1:200, overnight at 4 degree C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37 degree C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei)

product-image-AAA292050_IF15.jpg IF (Immunofluorescence) (Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37 degree C for 20 min;Incubation: Anti-NGL1 Polyclonal Antibody, Unconjugated(bs-6710R) 1:200, overnight at 4 degree C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37 degree C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
640
NCBI Official Full Name
Leucine-rich repeat-containing protein 4C
NCBI Official Synonym Full Names
leucine rich repeat containing 4C
NCBI Official Symbol
LRRC4C
NCBI Official Synonym Symbols
NGL1; NGL-1
NCBI Protein Information
leucine-rich repeat-containing protein 4C; netrin-G1 ligand
UniProt Protein Name
Leucine-rich repeat-containing protein 4C
UniProt Gene Name
LRRC4C
UniProt Synonym Gene Names
KIAA1580; NGL1; NGL-1
UniProt Entry Name
LRC4C_HUMAN

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Product Notes

The LRRC4C lrrc4c (Catalog #AAA292050) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Netrin G1 ligand Polyclonal Antibody reacts with Rat, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's Netrin G1 ligand can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the LRRC4C lrrc4c for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Netrin G1 ligand, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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