WB (Western Blot)
(Anti-Human NF2 (Merlin) pS518 Antibody - Western Blot. Affinity purified phospho-specific antibody to NF2 (Merlin) at pS518 was used at a 1:1000 dilution to detect NF2 by Western blot. Approximately 12 ul of a mouse cardiac myocyte lysate was loaded per lane on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock treated (lane 1) or CLA treated at 4nM, 20 nM or 100 nM (lanes 2, 3 and 4 respectively) for 45. After washing, a 1:5000 dilution of HRP conjugated Gt-a-Rabbit IgG preceded color development using Amershams substrate system. Other detection methods will yield similar results.)
Rabbit anti-Human, Mouse NF2 / Merlin Polyclonal Antibody | anti-NF2 antibody
Rabbit Polyclonal to Human NF2 / Merlin
Gene Names
NF2; ACN; SCH; BANF
Reactivity
Human, Mouse
Applications
ELISA, Immunoprecipitation, Western Blot, Immunohistochemistry
Purity
Immunoaffinity Purified
Synonyms
NF2 / Merlin, Antibody; Rabbit Polyclonal to Human NF2 / Merlin; Anti-NF2 / Merlin Antibody (phospho-Ser518) IHC-plus; NF2; ACN; BANF; Moesin-ezrin-radixin like; Neurofibromin 2 (merlin); Neurofibromin-2; SCH; Merlin; Schwannomerlin; Schwannomin; Human NF2; anti-NF2 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
Human NF2 (Merlin) phospho-peptide corresponding to a region of the human protein surrounding S518 and conjugated to Keyhole Limpet Hemocyanin (KLH).
Purity/Purification
Immunoaffinity Purified
Form/Format
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Concentration
1 mg/ml (varies by lot)
Sequence Length
595
Applicable Applications for anti-NF2 antibody
ELISA, IP (Immunoprecipitation), WB (Western Blot), IHC (Immunohistochemistry)
Target Species
Human
Antigen Modification
pSer518
Preparation and Storage
Store at -20 degree C or lower. Aliquot to avoid repeated freezing and thawing.
WB (Western Blot)
(Anti-Human NF2 (Merlin) pS518 Antibody - Western Blot. Affinity purified phospho-specific antibody to NF2 (Merlin) at pS518 was used at a 1:1000 dilution to detect NF2 by Western blot. Approximately 12 ul of a mouse cardiac myocyte lysate was loaded per lane on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock treated (lane 1) or CLA treated at 4nM, 20 nM or 100 nM (lanes 2, 3 and 4 respectively) for 45. After washing, a 1:5000 dilution of HRP conjugated Gt-a-Rabbit IgG preceded color development using Amershams substrate system. Other detection methods will yield similar results.)
WB (Western Blot)
(Anti-Human NF2 (Merlin) pS518 Antibody - Western Blot. Affinity purified phospho-specific antibody to NF2 (Merlin) at pS518 was used at a 1:1000 dilution to detect NF2 by Western blot. Approximately 12 ul of a mouse cardiac myocyte lysate was loaded per lane on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock treated (lane 1) or CLA treated at 4nM, 20 nM or 100 nM (lanes 2, 3 and 4 respectively) for 45. After washing, a 1:5000 dilution of HRP conjugated Gt-a-Rabbit IgG preceded color development using Amershams substrate system. Other detection methods will yield similar results.)
IHC (Immunohistochemistry)
(Anti-NF2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
IHC (Immunohistochemistry)
(Anti-NF2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Related Product Information for anti-NF2 antibody
Mutations linked to neurofibromatosis type 2 led to the identification of NF2. NF2 encodes a protein related to the cytoskeletal components moesin, ezrin, and radixin, and was thus named merlin.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
24,515 Da
NCBI Official Full Name
merlin isoform 1
NCBI Official Synonym Full Names
neurofibromin 2 (merlin)
NCBI Official Symbol
NF2
NCBI Official Synonym Symbols
ACN; SCH; BANF
NCBI Protein Information
merlin; schwannomin; schwannomerlin; neurofibromin-2; moesin-ezrin-radixin like; moesin-ezrin-radixin-like protein; moesin-ezrin-radizin-like protein; neurofibromin 2 (bilateral acoustic neuroma)
UniProt Protein Name
Merlin
UniProt Gene Name
NF2
UniProt Synonym Gene Names
SCH
UniProt Entry Name
MERL_HUMAN
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Product Notes
The NF2 nf2 (Catalog #AAA50741) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Polyclonal to Human NF2 / Merlin reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NF2 / Merlin can be used in a range of immunoassay formats including, but not limited to, ELISA, IP (Immunoprecipitation), WB (Western Blot), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the NF2 nf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NF2 / Merlin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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