Rabbit NFkappaB-p105/p50 Polyclonal Antibody | anti-NFkappaB-p105/p50 antibody

NFkappaB-p105/p50(Phospho-Ser337) Antibody

Cat. Num. AAA306251

• Gene Names: NFKB1; p50; KBF1; p105; EBP-1; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Synonyms: NFkappaB-p105/p50, Antibody; NFkappaB-p105/p50(Phospho-Ser337) Antibody; NFkB-p105/p50(Phospho-Ser337) Antibody; p50; KBF1; NF-kB1; NFKB-p50; NFkappaB; anti-NFkappaB-p105/p50 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of NFŽ degree B-p105/p50 only when phosphorylated at serine 337.
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 968

• Applicable Applications for anti-NFkappaB-p105/p50 antibody: IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen Type: Peptide-KLH
• Immunogen Description: Peptide sequence around phosphorylation site of serine 337(R-K-S(p)-D-L) derived from Human NFkappaB-p105/p50.
• Modification: Phospho-Ser337
• Target Name: NFkappaB-p105/p50
• Preparation and Storage: Store at -20 degree C

WB (Western Blot)

(Western blot analysis of extracts from Hela cells, treated with Noc or calf intestinal phosphatase (CIP), using NFkappaB-p105/p50(Phospho-Ser337) Antibody.)

WB (Western Blot)

(Western blot analysis of extract from HeLa cells, using NF-kappaB p105/p50 (phospho-Ser337) antibody (, Lane 1 and 2).)

IHC (Immunohiostchemistry)

(Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using NF-kappaB p105/p50 (phospho-Ser337) antibody ().)

IF (Immunofluorescence)

(Immunofluorescence staining of methanol-fixed HeLa cells using NF-kappaB p105/p50 (phospho-Ser337) antibody (, Red).)

Related Product Information for anti-NFkappaB-p105/p50 antibody

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and RelB-p50 complexes are transcriptional activators. The NF-kappa-B p50-p50 homodimer is a transcriptional repressor, but can act as a transcriptional activator when associated with BCL3. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslational processing. The proteasome-mediated process ensures the production of both p50 and p105 and preserves their independent function, although processing of NFKB1/p105 also appears to occur post-translationally. p50 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. In a complex with MAP3K8, NFKB1/p105 represses MAP3K8-induced MAPK signaling; active MAP3K8 is released by proteasome-dependent degradation of NFKB1/p105.

Product Categories/Family for anti-NFkappaB-p105/p50 antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 259155302
• NCBI GeneID: 4790
• NCBI Accession #: NP_001158884.1
• NCBI GenBank Nucleotide #: NM_001165412.1
• UniProt Accession #: P19838; Q68D84; Q86V43; Q8N4X7; Q9NZC0; A8K5Y5; B3KVE8
• Molecular Weight: 85,520 Da
• NCBI Official Full Name: nuclear factor NF-kappa-B p105 subunit isoform 2
• NCBI Official Synonym Full Names: nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
• NCBI Official Symbol: NFKB1
• NCBI Official Synonym Symbols: p50; KBF1; p105; EBP-1; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B
• NCBI Protein Information: nuclear factor NF-kappa-B p105 subunit
• UniProt Protein Name: Nuclear factor NF-kappa-B p105 subunit
• UniProt Gene Name: NFKB1
• UniProt Entry Name: NFKB1_HUMAN