WB (Western Blot)
(NFKBp65 Antibody (pS536) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the NFKBp65 antibody detected the NFKBp65 protein (arrow).)
Rabbit NFKBp65 Polyclonal Antibody | anti-RELA antibody
NFKBp65 Antibody (C-term S536)
Gene Names
RELA; p65; NFKB3
Reactivity
Human (Predicted Reactivity: Pig, Mouse)
Applications
Western Blot, ELISA, Immunofluorescence
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
NFKBp65, Antibody; NFKBp65 Antibody (C-term S536); Transcription factor p65; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; RELA; NFKB3; anti-RELA antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Pig, Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This NFKBp65 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 517-539 amino acids from the C-terminal region of human NFKBp65.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
517-539
Sequence Length
548
Applicable Applications for anti-RELA antibody
WB (Western Blot), ELISA, IF (Immunofluorescence)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(NFKBp65 Antibody (pS536) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the NFKBp65 antibody detected the NFKBp65 protein (arrow).)
WB (Western Blot)
(NFKBp65 Antibody (pS536) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the NFKBp65 antibody detected the NFKBp65 protein (arrow).)
IF (Immunofluorescence)
(Fluorescent image of U251 cell stained with NFKBp65 Antibody(C-termS536). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NFKBp65 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree).NFKBp65 immunoreactivity is localized to Cytoplasm significantly.)
IF (Immunofluorescence)
(Fluorescent image of U251 cell stained with NFKBp65 Antibody(C-termS536). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NFKBp65 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree).NFKBp65 immunoreactivity is localized to Cytoplasm significantly.)
Related Product Information for anti-RELA antibody
NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL
(MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex.
The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of
NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA,
MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by
trapping it in the cytoplasm. Phosphorylation of serine residues on
the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM
603258) marks them for destruction via the ubiquitination pathway,
thereby allowing activation of the NFKB complex. Activated NFKB
complex translocates into the nucleus and binds DNA at
kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or
5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G
purine; and Y is a C or T pyrimidine).
(MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex.
The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of
NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA,
MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by
trapping it in the cytoplasm. Phosphorylation of serine residues on
the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM
603258) marks them for destruction via the ubiquitination pathway,
thereby allowing activation of the NFKB complex. Activated NFKB
complex translocates into the nucleus and binds DNA at
kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or
5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G
purine; and Y is a C or T pyrimidine).
References
Pan, W.W., et al. J. Biol. Chem. 285(45):34348-34354(2010)
Tago, K., et al. J. Biol. Chem. 285(40):30622-30633(2010)
Park, J.S., et al. Oncol. Rep. 24(3):709-714(2010)
Yu, Z.H., et al. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 26(7):650-652(2010)
Rohwer, N., et al. PLoS ONE 5 (8), E12038 (2010) :
Tago, K., et al. J. Biol. Chem. 285(40):30622-30633(2010)
Park, J.S., et al. Oncol. Rep. 24(3):709-714(2010)
Yu, Z.H., et al. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 26(7):650-652(2010)
Rohwer, N., et al. PLoS ONE 5 (8), E12038 (2010) :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
60219
NCBI Official Full Name
transcription factor p65 isoform 2
NCBI Official Synonym Full Names
v-rel avian reticuloendotheliosis viral oncogene homolog A
NCBI Official Symbol
RELA
NCBI Official Synonym Symbols
p65; NFKB3
NCBI Protein Information
transcription factor p65
UniProt Protein Name
Transcription factor p65
UniProt Gene Name
RELA
UniProt Synonym Gene Names
NFKB3
UniProt Entry Name
TF65_HUMAN
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Product Notes
The RELA rela (Catalog #AAA283690) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 517-539. The NFKBp65 Antibody (C-term S536) reacts with Human (Predicted Reactivity: Pig, Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's NFKBp65 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA, IF (Immunofluorescence). Researchers should empirically determine the suitability of the RELA rela for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NFKBp65, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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