WB (Western Blot)
(Detection of human and mouse NIP7 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-NIP7 antibody AAA212420 (lot AAA212420-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human, Mouse NIP7 Polyclonal Antibody | anti-NIP7 antibody
Rabbit anti-NIP7 Antibody, Affinity Purified
Gene Names
NIP7; KD93; CGI-37; HSPC031
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
NIP7, Antibody; Rabbit anti-NIP7 Antibody, Affinity Purified; nuclear import 7 homolog; CGI-37; HSPC031; KD93; 60S ribosome subunit biogenesis protein NIP7 homolog; NIP7, nucleolar pre-rRNA processing protein; anti-NIP7 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-NIP7 antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Pig, Orangutan
Immunogen
Between 130 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse NIP7 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-NIP7 antibody AAA212420 (lot AAA212420-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human and mouse NIP7 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-NIP7 antibody AAA212420 (lot AAA212420-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human NIP7 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NIP7 antibody AAA212420 (lot AAA212420-1) used for IP at 6 ug/mg lysate. NIP7 was also immunoprecipitated by rabbit anti-NIP7 antibody BL14344. For blotting immunoprecipitated NIP7, AAA212420 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human NIP7 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NIP7 antibody AAA212420 (lot AAA212420-1) used for IP at 6 ug/mg lysate. NIP7 was also immunoprecipitated by rabbit anti-NIP7 antibody BL14344. For blotting immunoprecipitated NIP7, AAA212420 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Related Product Information for anti-NIP7 antibody
Nuclear import 7 homolog (NIP7) interacts with pre-ribosome complex and is required for proper 34S pre-rRNA processing and 60S ribosome subunit assembly [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9Y221].
Product Categories/Family for anti-NIP7 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
15,222 Da
NCBI Official Full Name
Homo sapiens NIP7, nucleolar pre-rRNA processing protein (NIP7), transcript variant 1, mRNA
NCBI Official Synonym Full Names
NIP7, nucleolar pre-rRNA processing protein
NCBI Official Symbol
NIP7
NCBI Official Synonym Symbols
KD93; CGI-37; HSPC031
NCBI Protein Information
60S ribosome subunit biogenesis protein NIP7 homolog; nuclear import 7 homolog
UniProt Protein Name
60S ribosome subunit biogenesis protein NIP7 homolog
UniProt Gene Name
NIP7
UniProt Entry Name
NIP7_HUMAN
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Product Notes
The NIP7 nip7 (Catalog #AAA212420) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-NIP7 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NIP7 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the NIP7 nip7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NIP7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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