WB (Western Blot)
(Detection of human NIR by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NIR antibody AAA212750 (lot AAA212750-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human NIR Polyclonal Antibody | anti-NIR antibody
Rabbit anti-NIR Antibody, Affinity Purified
Gene Names
NOC2L; NIR; NET7; NET15
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Antigen Affinity Purified
Synonyms
NIR, Antibody; Rabbit anti-NIR Antibody, Affinity Purified; novel INHAT (inhibitor of histone acetyltransferase) repressor; protein phosphatase 1, regulatory subunit 12; protein NOC2 homolog; PPP1R112; nucleolar complex protein 2 homolog; novel INHAT repressor; NOC2-like protein; NOC2L nucleolar associated transcriptional repressor; NIR; NET7; NET15; nucleolar complex associated 2 homolog; Nucleolar complex protein 2 homolog; NOC2 like nucleolar associated transcriptional repressor; anti-NIR antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-NIR antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
Between 699 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human NIR by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NIR antibody AAA212750 (lot AAA212750-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human NIR by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NIR antibody AAA212750 (lot AAA212750-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IHC (Immunohistochemistry)
(Detection of human NIR by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-NIR (Cat. No. AAA212750 Lot1) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).)
IHC (Immunohistochemistry)
(Detection of human NIR by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-NIR (Cat. No. AAA212750 Lot1) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).)
Related Product Information for anti-NIR antibody
Novel INHAT repressor (NIR) acts as an inhibitor of histone acetyltransferase activity; it prevents acetylation of all core histones by the EP300/p300 histone acetyltransferase at p53/TP53-regulated target promoters in a histone deacetylases (HDAC)-independent manner. NIR acts as a transcription corepressor of p53/TP53- and TP63-mediated transactivation of the p21/CDKN1A promoter. It is involved in the regulation of p53/TP53-dependent apoptosis and it associates together with TP63 isoform TA*-gamma to the p21/CDKN1A promoter [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9Y3T9].
Product Categories/Family for anti-NIR antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
84,919 Da
NCBI Official Full Name
nucleolar complex protein 2 homolog
NCBI Official Synonym Full Names
nucleolar complex associated 2 homolog (S. cerevisiae)
NCBI Official Symbol
NOC2L
NCBI Official Synonym Symbols
NIR; NET7; NET15
NCBI Protein Information
nucleolar complex protein 2 homolog; NOC2-like protein; protein NOC2 homolog; novel INHAT repressor; novel INHAT (inhibitor of histone acetyltransferase) repressor
UniProt Protein Name
Nucleolar complex protein 2 homolog
UniProt Gene Name
NOC2L
UniProt Synonym Gene Names
NIR; Protein NOC2 homolog
UniProt Entry Name
NOC2L_HUMAN
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Product Notes
The NIR noc2l (Catalog #AAA212750) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-NIR Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NIR can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the NIR noc2l for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NIR, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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