IHC (Immunohistochemisry)
(Mouse Nlk Antibody (C-term) (AAA288115)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Nlk Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Rabbit Nlk Polyclonal Antibody | anti-Nlk antibody
Mouse Nlk Antibody (C-term)
Gene Names
Nlk; AI194375
Reactivity
Human, mouse (Predicted Reactivity: Bovine, Rat)
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Nlk, Antibody; Mouse Nlk Antibody (C-term); Serine/threonine-protein kinase NLK; Nemo-like kinase; Nlk {ECO:0000312|EMBL:AAC244991}; anti-Nlk antibody
Host
Rabbit
Reactivity
Human, mouse (Predicted Reactivity: Bovine, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This Mouse Nlk antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 408-436 amino acids from the C-terminal region of mouse Nlk.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
408-436
Sequence Length
527
Applicable Applications for anti-Nlk antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
MOUSE
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistochemisry)
(Mouse Nlk Antibody (C-term) (AAA288115)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Nlk Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohistochemisry)
(Mouse Nlk Antibody (C-term) (AAA288115)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Nlk Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(Mouse Nlk Antibody (C-term) western blot analysis in mouse lung tissue lysates (35ug/lane).This demonstrates the Nlk antibody detected the Nlk protein (arrow).)
WB (Western Blot)
(Mouse Nlk Antibody (C-term) western blot analysis in mouse lung tissue lysates (35ug/lane).This demonstrates the Nlk antibody detected the Nlk protein (arrow).)
WB (Western Blot)
(Mouse Nlk Antibody (C-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the Nlk antibody detected the Nlk protein (arrow).)
WB (Western Blot)
(Mouse Nlk Antibody (C-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the Nlk antibody detected the Nlk protein (arrow).)
Related Product Information for anti-Nlk antibody
Role in cell fate determination, required for differentiation of bone marrow stromal cells. Acts downstream of MAP3K7 and HIPK2 to negatively regulate the canonical Wnt/beta-catenin signaling pathway and the phosphorylation and destruction of the MYB transcription factor. May suppress a wide range of transcription factors by phosphorylation of the coactivator, CREBBP. Involved in TGFbeta-mediated mesoderm induction, acting downstream of MAP3K7/TAK1 to phosphorylate STAT3.
Product Categories/Family for anti-Nlk antibody
References
Li, M., et al. J. Biol. Chem. 285(18):13397-13404(2010)
Nifuji, A., et al. Exp. Cell Res. 316(7):1127-1136(2010)
Ishitani, T., et al. Nat. Cell Biol. 12(3):278-285(2010)
Martinez, G., et al. Invest. Ophthalmol. Vis. Sci. 50(10):4794-4806(2009)
Harwood, B.N., et al. Dev. Dyn. 237(4):1099-1111(2008)
Nifuji, A., et al. Exp. Cell Res. 316(7):1127-1136(2010)
Ishitani, T., et al. Nat. Cell Biol. 12(3):278-285(2010)
Martinez, G., et al. Invest. Ophthalmol. Vis. Sci. 50(10):4794-4806(2009)
Harwood, B.N., et al. Dev. Dyn. 237(4):1099-1111(2008)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
58313
NCBI Official Full Name
serine/threonine-protein kinase NLK
NCBI Official Synonym Full Names
nemo like kinase
NCBI Official Symbol
Nlk
NCBI Official Synonym Symbols
AI194375
NCBI Protein Information
serine/threonine-protein kinase NLK
UniProt Protein Name
Serine/threonine-protein kinase NLK
UniProt Gene Name
Nlk
UniProt Entry Name
NLK_MOUSE
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Product Notes
The Nlk nlk (Catalog #AAA288115) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 408-436. The Mouse Nlk Antibody (C-term) reacts with Human, mouse (Predicted Reactivity: Bovine, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's Nlk can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the Nlk nlk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nlk, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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