IF (Immunofluorescence)
(Confocal immunofluorescent analysis of NLK-T286 Antibody (Center) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
Rabbit NLK-T286 Polyclonal Antibody | anti-NLK antibody
NLK-T286 Antibody (Center)
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Rat)
Applications
Immunofluorescence, Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
NLK-T286, Antibody; NLK-T286 Antibody (Center); Serine/threonine-protein kinase NLK; Nemo-like kinase; Protein LAK1; NLK; LAK1 {ECO:0000312|EMBL:AAD560131}; anti-NLK antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This NLK-T286 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 275-300 amino acids from the Central region of human NLK-T286.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
275-300
Sequence Length
527
Applicable Applications for anti-NLK antibody
IF (Immunofluorescence), FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of NLK-T286 Antibody (Center) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of NLK-T286 Antibody (Center) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
FCM/FACS (Flow Cytometry)
(NLK-T286 Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(NLK-T286 Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(NLK-T286 western blot analysis in Y79 cell line lysates (35ug/lane).This demonstrates the NLK antibody detected the NLK protein (arrow).)
WB (Western Blot)
(NLK-T286 western blot analysis in Y79 cell line lysates (35ug/lane).This demonstrates the NLK antibody detected the NLK protein (arrow).)
Product Categories/Family for anti-NLK antibody
References
Kim, S., et al. J. Biol. Chem. 285(11):8122-8129(2010)
Ishitani, T., et al. Nat. Cell Biol. 12(3):278-285(2010)
Shi, Y., et al. Mol. Cell. Biochem. 333 (1-2), 293-298 (2010) :
Emami, K.H., et al. Prostate 69(14):1481-1492(2009)
Saijo, K., et al. Cell 137(1):47-59(2009)
Wistow, G., et al. Mol. Vis. 8, 196-204 (2002) :
Kortenjann, M., et al. Gene 278 (1-2), 161-165 (2001) :
Kehrer-Sawatzki, H., et al. Gene 251(1):63-71(2000)
Brott, B.K., et al. Proc. Natl. Acad. Sci. U.S.A. 95(3):963-968(1998)
Volorio, S., et al. DNA Seq. 9 (5-6), 307-315 (1998) :
Ishitani, T., et al. Nat. Cell Biol. 12(3):278-285(2010)
Shi, Y., et al. Mol. Cell. Biochem. 333 (1-2), 293-298 (2010) :
Emami, K.H., et al. Prostate 69(14):1481-1492(2009)
Saijo, K., et al. Cell 137(1):47-59(2009)
Wistow, G., et al. Mol. Vis. 8, 196-204 (2002) :
Kortenjann, M., et al. Gene 278 (1-2), 161-165 (2001) :
Kehrer-Sawatzki, H., et al. Gene 251(1):63-71(2000)
Brott, B.K., et al. Proc. Natl. Acad. Sci. U.S.A. 95(3):963-968(1998)
Volorio, S., et al. DNA Seq. 9 (5-6), 307-315 (1998) :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
58283
NCBI Official Full Name
serine/threonine-protein kinase NLK
NCBI Official Synonym Full Names
nemo-like kinase
NCBI Official Symbol
NLK
NCBI Protein Information
serine/threonine-protein kinase NLK
UniProt Protein Name
Serine/threonine-protein kinase NLK
UniProt Gene Name
NLK
UniProt Entry Name
NLK_HUMAN
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Product Notes
The NLK nlk (Catalog #AAA289492) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 275-300. The NLK-T286 Antibody (Center) reacts with Human (Predicted Reactivity: Bovine, Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's NLK-T286 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the NLK nlk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NLK-T286, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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