Rabbit NLRP3 Polyclonal Antibody | anti-NLRP3 antibody

NLRP3 Polyclonal Antibody

Cat. Num. AAA30558

• Gene Names: NLRP3; AII; AVP; FCU; MWS; FCAS; CIAS1; NALP3; C1orf7; CLR1.1; PYPAF1; AGTAVPRL; FLJ95925
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity purification
• Synonyms: NLRP3; Polyclonal Antibody; NLRP3 Polyclonal Antibody; AGTAVPRL; AII; AVP; C1orf7; CIAS1; CLR1.1; FCAS; FCAS1; FCU; MWS; NALP3; PYPAF1; anti-NLRP3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purification
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH7.4, 150mM NaCl, 0.02% sodium azide and 239% glycerol.; PBS with 0.02% sodium azide, 50% glycerol, pH7.192
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-NLRP3 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:1000-1:3000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: A synthetic peptide of human NLRP3 (NP_001120934.1)
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

WB (Western Blot)

(Western blot analysis of extracts of U-937 cells, using NLRP3 antibody at 1:1000 dilution.)

IHC (Immunohistchemistry)

(Immunohistochemistry of paraffin-embedded mouse spleen using NLRP3 antibody at dilution of 1:100 .)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded human appendix using NLRP3 antibody at dilution of 1:100 .)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded rat lung using NLRP3 antibody at dilution of 1:100 .)

IF (Immunofluorescence)

(Immunofluorescence analysis of rat lung cells using NLRP3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence)

(Immunofluorescence analysis of Raw264.7 cells using NLRP3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence)

(Immunofluorescence analysis of Raw264.7 cells using NLRP3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

Related Product Information for anti-NLRP3 antibody

This gene encodes a pyrin-like protein containing a pyrin domain, a nucleotide-binding site (NBS) domain, and a leucine-rich repeat (LRR) motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, and is a member of the NALP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling, and it plays a role in the regulation of inflammation, the immune response, and apoptosis. Mutations in this gene are associated with familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous and articular (CINCA) syndrome, and neonatal-onset multisystem inflammatory disease (NOMID). Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Alternative 5' UTR structures are suggested by available data; however, insufficient evidence is available to determine if all of the represented 5' UTR splice patterns are biologically valid.

Product Categories/Family for anti-NLRP3 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 188536002
• NCBI GeneID: 114548
• NCBI Accession #: NP_001120934.1
• NCBI GenBank Nucleotide #: NM_001127462.2
• UniProt Accession #: Q96P20; Q65Z67
• Molecular Weight: 6,129 Da
• NCBI Official Full Name: NACHT, LRR and PYD domains-containing protein 3 isoform d
• NCBI Official Synonym Full Names: NLR family, pyrin domain containing 3
• NCBI Official Symbol: NLRP3
• NCBI Official Synonym Symbols: AII; AVP; FCU; MWS; FCAS; CIAS1; NALP3; C1orf7; CLR1.1; PYPAF1; AGTAVPRL; FLJ95925
• NCBI Protein Information: NACHT, LRR and PYD domains-containing protein 3; cryopyrin; OTTHUMP00000038408; OTTHUMP00000038409; OTTHUMP00000038410; OTTHUMP00000038411; OTTHUMP00000230825; caterpiller protein 1.1; PYRIN-containing APAF1-like protein 1; NACHT, LRR and PYD containing p
• UniProt Protein Name: NACHT, LRR and PYD domains-containing protein 3
• UniProt Gene Name: NLRP3
• UniProt Synonym Gene Names: C1orf7; CIAS1; NALP3; PYPAF1; CLR1.1
• UniProt Entry Name: NALP3_HUMAN