IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Rabbit anti-Human NM23 (NME1) Polyclonal Antibody | anti-NME1 antibody
NM23 (NME1) Antibody (N-term)
Gene Names
NME1; NB; AWD; NBS; GAAD; NDKA; NM23; NDPKA; NDPK-A; NM23-H1
Reactivity
Human
Applications
Immunohistochemistry, Immunofluorescence, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
NM23 (NME1), Antibody; NM23 (NME1) Antibody (N-term); Nucleoside diphosphate kinase A; NDK A; NDP kinase A; Granzyme A-activated DNase; GAAD; Metastasis inhibition factor nm23; NM23-H1; Tumor metastatic process-associated protein; NME1; NDPKA; NM23; anti-NME1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This NM23 (NME1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 25-54 amino acids from the N-terminal region of human NM23 (NME1).
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
25-54
Sequence Length
267
Applicable Applications for anti-NME1 antibody
IHC (Immunohistochemistry), IF (Immunofluorescence), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of NM23 (NME1) Antibody (N-term) with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of NM23 (NME1) Antibody (N-term) with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
WB (Western Blot)
(NME1 Antibody (F40) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the NME1 antibody detected the NME1 protein (arrow).)
WB (Western Blot)
(NME1 Antibody (F40) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the NME1 antibody detected the NME1 protein (arrow).)
Related Product Information for anti-NME1 antibody
NME1 was identified because of its reduced mRNA transcript levels in highly metastatic cells. NME1 encodes the 'A' isoform of nucleoside diphosphate kinase (NDK). NDK exists as a hexamer composed of the 'A' (NME1) and 'B' (encoded by NME2) isoforms. Mutations in NME1 have been identified in aggressive neuroblastomas.
References
Munier, A., et al., Exp. Cell Res. 289(2):295-306 (2003).
Chen, Y., et al., J. Mol. Biol. 332(4):915-926 (2003).
Kim, Y.I., et al., Biochem. Biophys. Res. Commun. 307(2):281-289 (2003).
Wang, P.H., et al., Gynecol. Obstet. Invest. 55(1):14-19 (2003).
Ni, X., et al., J. Hum. Genet. 48(2):96-100 (2003).
Chen, Y., et al., J. Mol. Biol. 332(4):915-926 (2003).
Kim, Y.I., et al., Biochem. Biophys. Res. Commun. 307(2):281-289 (2003).
Wang, P.H., et al., Gynecol. Obstet. Invest. 55(1):14-19 (2003).
Ni, X., et al., J. Hum. Genet. 48(2):96-100 (2003).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17149
NCBI Official Full Name
nucleoside diphosphate kinase A isoform b
NCBI Official Synonym Full Names
NME/NM23 nucleoside diphosphate kinase 1
NCBI Official Symbol
NME1
NCBI Official Synonym Symbols
NB; AWD; NBS; GAAD; NDKA; NM23; NDPKA; NDPK-A; NM23-H1
NCBI Protein Information
nucleoside diphosphate kinase A
UniProt Protein Name
Nucleoside diphosphate kinase A
UniProt Gene Name
NME1
UniProt Synonym Gene Names
NDPKA; NM23; NDK A; NDP kinase A; GAAD
UniProt Entry Name
NDKA_HUMAN
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Product Notes
The NME1 nme1 (Catalog #AAA284387) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 25-54. The NM23 (NME1) Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NM23 (NME1) can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IF (Immunofluorescence), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the NME1 nme1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NM23 (NME1), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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