ICC (Immunocytochemistry)
(Anti-NM23A antibody, AAA45483, ICCICC: HELA Cell)
Rabbit NM23A Polyclonal Antibody | anti-NME1 antibody
Anti-NM23A antibody
Gene Names
NME1; NB; AWD; NBS; GAAD; NDKA; NM23; NDPKA; NDPK-A; NM23-H1
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Synonyms
NM23A, Antibody; Anti-NM23A antibody; Nucleoside diphosphate kinase A(NDK A); NME/NM23 nucleoside diphosphate kinase 1; AWD antibody; AWD, drosophila, homolog of antibody; GAAD antibody; Granzyme A activated DNase antibody; Granzyme A-activated DNase antibody; GZMA activated DNase antibody; Metastasis inhibition factor NM23 antibody; NB antibody; NBS antibody; NDK A antibody; NDKA antibody; NDKA_HUMAN antibody; NDP kinase A antibody; NDPK-A antibody; NDPKA antibody; NM23 antibody; NM23 long variant, included antibody; nm23-H1 antibody; NM23-M1 antibody; NM23H1B, included antibody; NME/NM23 nucleoside diphosphate kinase 1 antibody; Nme1 antibody; NME1-NME2 spliced read-through transcript, included antibody; Non-metastatic cells 1, protein(NM23A) expressed in antibody; Nonmetastatic cells 1, protein expressed in antibody; Nonmetastatic protein 23 antibody; Nonmetastatic protein 23, homolog 1 antibody; Nucleoside diphosphate kinase A antibody; Tumor metastatic process-associated protein antibody; anti-NME1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
152
Applicable Applications for anti-NME1 antibody
ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human NM23A (137-152aa EELVDYTSCAQNWIYE), different from the related mouse sequence by two amino acids and from rat sequence by one amino acid.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
ICC (Immunocytochemistry)
(Anti-NM23A antibody, AAA45483, ICCICC: HELA Cell)
ICC (Immunocytochemistry)
(Anti-NM23A antibody, AAA45483, ICCICC: HELA Cell)
IHC (Immunohiostchemistry)
(Anti-NM23A antibody, AAA45483, IHC(P)IHC(P): Rat Cerebellum Tissue)
IHC (Immunohiostchemistry)
(Anti-NM23A antibody, AAA45483, IHC(P)IHC(P): Rat Cerebellum Tissue)
WB (Western Blot)
(Anti-NM23A antibody, AAA45483, Western blottingLane 1: Rat Heart Tissue LysateLane 2: Rat Brain Tissue LysateLane 3: Rat Liver Tissue LysateLane 4: Rat Skeletal Muscle Tissue LysateLane 5: PANC Cell LysateLane 6: HELA Cell LysateLane 7: SKOV Cell LysateLane 8: COLO320 Cell Lysate)
WB (Western Blot)
(Anti-NM23A antibody, AAA45483, Western blottingLane 1: Rat Heart Tissue LysateLane 2: Rat Brain Tissue LysateLane 3: Rat Liver Tissue LysateLane 4: Rat Skeletal Muscle Tissue LysateLane 5: PANC Cell LysateLane 6: HELA Cell LysateLane 7: SKOV Cell LysateLane 8: COLO320 Cell Lysate)
Related Product Information for anti-NME1 antibody
Description: Rabbit IgG polyclonal antibody for Nucleoside diphosphate kinase A(NME1) detection. Tested with WB, IHC-P, ICC in Human, Mouse, Rat.
Background: NME1(NME/NM23 nucleoside diphosphate kinase 1) also called non-metastatic cells 1, protein(NM23A) expressed in,NM23, NM23-H1, NDPKA, GAAD or AWD, is an enzyme that in humans is encoded by the NME1 gene. The promoters of the mouse and human NME1 genes, like those of other NME genes, contain several binding sites for AP2, NF1, Sp1, LEF1, and response elements to glucocorticoid receptors. The NME1 gene is mapped on 17q21.33. Immunofluorescence microscopy demonstrated colocalization of NME1 in nuclei of B cells expressing EBNA3C. Expression of EBNA3C reversed the ability of NME1 to inhibit migration of BL and breast carcinoma cells. NM23H1 bound SET and was released from inhibition by GZMA cleavage of SET. After GZMA loading or cytotoxic T lymphocyte attack, SET and NM23H1 translocated to the nucleus and SET was degraded, allowing NM23H1 to nick chromosomal DNA. Using a Drosophila model system, Dammai et al.(2003) showed that the Drosophila NME1 homolog, awd, regulates trachea cell motility by modulating FGFR levels through a dynamin -mediated pathway.
Background: NME1(NME/NM23 nucleoside diphosphate kinase 1) also called non-metastatic cells 1, protein(NM23A) expressed in,NM23, NM23-H1, NDPKA, GAAD or AWD, is an enzyme that in humans is encoded by the NME1 gene. The promoters of the mouse and human NME1 genes, like those of other NME genes, contain several binding sites for AP2, NF1, Sp1, LEF1, and response elements to glucocorticoid receptors. The NME1 gene is mapped on 17q21.33. Immunofluorescence microscopy demonstrated colocalization of NME1 in nuclei of B cells expressing EBNA3C. Expression of EBNA3C reversed the ability of NME1 to inhibit migration of BL and breast carcinoma cells. NM23H1 bound SET and was released from inhibition by GZMA cleavage of SET. After GZMA loading or cytotoxic T lymphocyte attack, SET and NM23H1 translocated to the nucleus and SET was degraded, allowing NM23H1 to nick chromosomal DNA. Using a Drosophila model system, Dammai et al.(2003) showed that the Drosophila NME1 homolog, awd, regulates trachea cell motility by modulating FGFR levels through a dynamin -mediated pathway.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
30,137 Da
NCBI Official Full Name
Nucleoside diphosphate kinase A
NCBI Official Synonym Full Names
NME/NM23 nucleoside diphosphate kinase 1
NCBI Official Symbol
NME1
NCBI Official Synonym Symbols
NB; AWD; NBS; GAAD; NDKA; NM23; NDPKA; NDPK-A; NM23-H1
NCBI Protein Information
nucleoside diphosphate kinase A; NDP kinase A; granzyme A-activated DNase; metastasis inhibition factor nm23; tumor metastatic process-associated protein; non-metastatic cells 1, protein (NM23A) expressed in
UniProt Protein Name
Nucleoside diphosphate kinase A
UniProt Gene Name
NME1
UniProt Synonym Gene Names
NDPKA; NM23; NDK A; NDP kinase A; GAAD
UniProt Entry Name
NDKA_HUMAN
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Product Notes
The NME1 nme1 (Catalog #AAA45483) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-NM23A antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NM23A can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the NME1 nme1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NM23A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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