IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded H.liver tissue reacted with NMNAT3 Antibody (Center))
Rabbit anti-Human NMNAT3 Polyclonal Antibody | anti-NMNAT3 antibody
NMNAT3 Antibody (Center)
Gene Names
NMNAT3; PNAT3; FKSG76; PNAT-3
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
NMNAT3, Antibody; NMNAT3 Antibody (Center); Nicotinamide mononucleotide adenylyltransferase 3; NMN adenylyltransferase 3; Nicotinate-nucleotide adenylyltransferase 3; NaMN adenylyltransferase 3; Pyridine nucleotide adenylyltransferase 3; PNAT-3; NMNAT3; anti-NMNAT3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This NMNAT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 150-178 amino acids from the Central region of human NMNAT3.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
150-178
Sequence Length
163
Applicable Applications for anti-NMNAT3 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Function
Catalyzes the formation of NAD(+) from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate with the same efficiency. Can use triazofurin monophosphate (TrMP) as substrate. Can also use GTP and ITP as nucleotide donors. Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD(+). For the pyrophosphorolytic activity, can use NAD(+), NADH, NaAD, nicotinic acid adenine dinucleotide phosphate (NHD), nicotinamide guanine dinucleotide (NGD) as substrates. Fails to cleave phosphorylated dinucleotides NADP(+), NADPH and NaADP(+). Protects against axonal degeneration following injury.
Cellular Location
Mitochondrion
Tissue Location
Expressed in lung and spleen with lower levels in placenta and kidney.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded H.liver tissue reacted with NMNAT3 Antibody (Center))
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded H.liver tissue reacted with NMNAT3 Antibody (Center))
WB (Western Blot)
(NMNAT3 Antibody (Center) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the NMNAT3 antibody detected the NMNAT3 protein (arrow).)
WB (Western Blot)
(NMNAT3 Antibody (Center) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the NMNAT3 antibody detected the NMNAT3 protein (arrow).)
Related Product Information for anti-NMNAT3 antibody
The coenzyme NAD and its derivatives are involved in
hundreds of metabolic redox reactions and are utilized in protein
ADP-ribosylation, histone deacetylation, and in some Ca(2+)
signaling pathways. NMNAT (EC 2.7.7.1) is a central enzyme in NAD
biosynthesis, catalyzing the condensation of nicotinamide
mononucleotide (NMN) or nicotinic acid mononucleotide (NaMN) with
the AMP moiety of ATP to form NAD or NaAD (Zhang et al., 2003
[PubMed 12574164]).
hundreds of metabolic redox reactions and are utilized in protein
ADP-ribosylation, histone deacetylation, and in some Ca(2+)
signaling pathways. NMNAT (EC 2.7.7.1) is a central enzyme in NAD
biosynthesis, catalyzing the condensation of nicotinamide
mononucleotide (NMN) or nicotinic acid mononucleotide (NaMN) with
the AMP moiety of ATP to form NAD or NaAD (Zhang et al., 2003
[PubMed 12574164]).
Product Categories/Family for anti-NMNAT3 antibody
References
Di Stefano, M., et al. Blood Cells Mol. Dis. 45(1):33-39(2010)
Lau, C., et al. J. Biol. Chem. 285(24):18868-18876(2010)
Sorci, L., et al. Biochemistry 46(16):4912-4922(2007)
Berger, F., et al. J. Biol. Chem. 280(43):36334-36341(2005)
Magni, G., et al. Cell. Mol. Life Sci. 61(1):19-34(2004)
Lau, C., et al. J. Biol. Chem. 285(24):18868-18876(2010)
Sorci, L., et al. Biochemistry 46(16):4912-4922(2007)
Berger, F., et al. J. Biol. Chem. 280(43):36334-36341(2005)
Magni, G., et al. Cell. Mol. Life Sci. 61(1):19-34(2004)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28322
NCBI Official Full Name
nicotinamide/nicotinic acid mononucleotide adenylyltransferase 3 isoform 2
NCBI Official Synonym Full Names
nicotinamide nucleotide adenylyltransferase 3
NCBI Official Symbol
NMNAT3
NCBI Official Synonym Symbols
PNAT3; FKSG76; PNAT-3
NCBI Protein Information
nicotinamide/nicotinic acid mononucleotide adenylyltransferase 3
UniProt Protein Name
Nicotinamide/nicotinic acid mononucleotide adenylyltransferase 3
UniProt Gene Name
NMNAT3
UniProt Synonym Gene Names
NMN/NaMN adenylyltransferase 3; NMN adenylyltransferase 3; NaMN adenylyltransferase 3; PNAT-3
UniProt Entry Name
NMNA3_HUMAN
Similar Products
Product Notes
The NMNAT3 nmnat3 (Catalog #AAA285838) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 150-178. The NMNAT3 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NMNAT3 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the NMNAT3 nmnat3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NMNAT3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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