FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of HL60 cells using Anti-NOS1AP Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Rabbit IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.)
Rabbit anti-Human NOS1AP Polyclonal Antibody | anti-NOS1AP antibody
Anti-NOS1AP Antibody
Gene Names
NOS1AP; CAPON; 6330408P19Rik
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Synonyms
NOS1AP, Antibody; Anti-NOS1AP Antibody; anti-NOS1AP antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of NOS1AP protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH7.3, 30% glycerol, and 0.01% sodium azide.
Applicable Applications for anti-NOS1AP antibody
FCM/FACS (Flow Cytometry), WB (Western Blot)
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the Central region of human NOS1AP. The exact sequence is proprietary.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of HL60 cells using Anti-NOS1AP Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Rabbit IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of HL60 cells using Anti-NOS1AP Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Rabbit IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.)
WB (Western Blot)
(Western blot analysis of NOS1AP expression in MCF7 (A) whole cell lysates. (Predicted band size: 56 kD; Observed band size: 21 kD))
WB (Western Blot)
(Western blot analysis of NOS1AP expression in MCF7 (A) whole cell lysates. (Predicted band size: 56 kD; Observed band size: 21 kD))
Related Product Information for anti-NOS1AP antibody
Rabbit polyclonal antibody to NOS1AP
Product Categories/Family for anti-NOS1AP antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
55,569 Da
NCBI Official Full Name
carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase protein isoform 2
NCBI Official Synonym Full Names
nitric oxide synthase 1 (neuronal) adaptor protein
NCBI Official Symbol
NOS1AP
NCBI Official Synonym Symbols
CAPON; 6330408P19Rik
NCBI Protein Information
carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase protein; nitric oxide synthase 1 adaptor protein; C-terminal PDZ ligand of neuronal nitric oxide synthase protein; C-terminal PDZ domain ligand of neuronal nitric oxide synthase (CAPON); ligan
UniProt Protein Name
Carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase protein
UniProt Gene Name
NOS1AP
UniProt Synonym Gene Names
CAPON; KIAA0464
UniProt Entry Name
CAPON_HUMAN
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Product Notes
The NOS1AP nos1ap (Catalog #AAA265255) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-NOS1AP Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NOS1AP can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), WB (Western Blot). Researchers should empirically determine the suitability of the NOS1AP nos1ap for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NOS1AP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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