FCM/FACS (Flow Cytometry)
(NOS1AP Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit NOS1AP Polyclonal Antibody | anti-NOS1AP antibody
NOS1AP Antibody (Center)
Gene Names
NOS1AP; CAPON; 6330408P19Rik
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Applications
Western Blot, ELISA, Flow Cytometry, Functional Assay
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
NOS1AP, Antibody; NOS1AP Antibody (Center); Carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase protein; C-terminal PDZ ligand of neuronal nitric oxide synthase protein; Nitric oxide synthase 1 adaptor protein; NOS1AP; CAPON; KIAA0464; anti-NOS1AP antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This NOS1AP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 437-466 amino acids from the Central region of human NOS1AP.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
437-466
Sequence Length
211
Applicable Applications for anti-NOS1AP antibody
WB (Western Blot), ELISA, FCM/FACS (Flow Cytometry)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(NOS1AP Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(NOS1AP Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(Western blot analysis of lysate from MCF-7 cell line, using NOS1AP Antibody (Center). AAA286487 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.)
WB (Western Blot)
(Western blot analysis of lysate from MCF-7 cell line, using NOS1AP Antibody (Center). AAA286487 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.)
Related Product Information for anti-NOS1AP antibody
This gene encodes a cytosolic protein that binds to the
signaling molecule, neuronal nitric oxide synthase (nNOS). This
protein has a C-terminal PDZ-binding domain that mediates
interactions with nNOS and an N-terminal phosphotyrosine binding
(PTB) domain that binds to the small monomeric G protein, Dexras1.
Studies of the related mouse and rat proteins have shown that this
protein functions as an adapter protein linking nNOS to specific
targets, such as Dexras1 and the synapsins. Alternative splicing
results in multiple transcript variants encoding different
isoforms.
signaling molecule, neuronal nitric oxide synthase (nNOS). This
protein has a C-terminal PDZ-binding domain that mediates
interactions with nNOS and an N-terminal phosphotyrosine binding
(PTB) domain that binds to the small monomeric G protein, Dexras1.
Studies of the related mouse and rat proteins have shown that this
protein functions as an adapter protein linking nNOS to specific
targets, such as Dexras1 and the synapsins. Alternative splicing
results in multiple transcript variants encoding different
isoforms.
Product Categories/Family for anti-NOS1AP antibody
References
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Lu, J., et al. Diabet. Med. 27(9):1074-1079(2010)
Husted, J.A., et al. Schizophr. Res. 121 (1-3), 187-192 (2010) :
Tomas, M., et al. J. Am. Coll. Cardiol. 55(24):2745-2752(2010)
Delorme, R., et al. BMC Med. Genet. 11, 108 (2010) :
Lu, J., et al. Diabet. Med. 27(9):1074-1079(2010)
Husted, J.A., et al. Schizophr. Res. 121 (1-3), 187-192 (2010) :
Tomas, M., et al. J. Am. Coll. Cardiol. 55(24):2745-2752(2010)
Delorme, R., et al. BMC Med. Genet. 11, 108 (2010) :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
56150
NCBI Official Full Name
carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase protein isoform 2
NCBI Official Synonym Full Names
nitric oxide synthase 1 (neuronal) adaptor protein
NCBI Official Symbol
NOS1AP
NCBI Official Synonym Symbols
CAPON; 6330408P19Rik
NCBI Protein Information
carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase protein
UniProt Protein Name
Carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase protein
UniProt Gene Name
NOS1AP
UniProt Synonym Gene Names
CAPON; KIAA0464
UniProt Entry Name
CAPON_HUMAN
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Product Notes
The NOS1AP nos1ap (Catalog #AAA286487) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 437-466. The NOS1AP Antibody (Center) reacts with Human (Predicted Reactivity: Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's NOS1AP can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the NOS1AP nos1ap for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NOS1AP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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