WB (Western Blot)
(Detection of human Notch2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Notch2 antibody AAA211759 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Notch2 was also immunoprecipitated by rabbit anti-Notch2 antibody which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).)
Rabbit anti-Human Notch2 Polyclonal Antibody | anti-Notch2 antibody
Rabbit anti-Notch2 Antibody, Affinity Purified
Gene Names
NOTCH2; hN2; AGS2; HJCYS
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
Notch2, Antibody; Rabbit anti-Notch2 Antibody, Affinity Purified; AGS2; Notch homolog 2; Notch 2; neurogenic locus notch homolog protein 2; HJCYS; hN2; Neurogenic locus notch homolog protein 2; notch 2; anti-Notch2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-Notch2 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 2275 and 2325
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human Notch2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Notch2 antibody AAA211759 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Notch2 was also immunoprecipitated by rabbit anti-Notch2 antibody which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).)
WB (Western Blot)
(Detection of human Notch2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Notch2 antibody AAA211759 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Notch2 was also immunoprecipitated by rabbit anti-Notch2 antibody which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).)
IHC (Immunohiostchemistry)
(Detection of human Notch2 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Notch2 (Cat. No. AAA211759 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
IHC (Immunohiostchemistry)
(Detection of human Notch2 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Notch2 (Cat. No. AAA211759 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
FCM/FACS (Flow Cytometry)
(Detection of human Notch2 (shaded) in U931 cells by flow cytometry. Antibody: Rabbit anti-Notch2 polyclonal antibody (AAA211759) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
FCM/FACS (Flow Cytometry)
(Detection of human Notch2 (shaded) in U931 cells by flow cytometry. Antibody: Rabbit anti-Notch2 polyclonal antibody (AAA211759) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
Related Product Information for anti-Notch2 antibody
Notch2 is a member of the Notch family of transmembrane receptors. The Notch gene was first reported as a drosophila gene responsible for the presence of notches in the wingblades of a specific strain of flies. The Notch proteins mediate juxtacrine signals between adjacent cells and regulate cell fate determination during development. In mammals there are 4 Notch receptors (Notch 1-4). Like Notch1, Notch 2 is required for embryo viability. Although Notch1 and Notch 2 are functionally similar, they may not serve redundant functions in the cell.
Product Categories/Family for anti-Notch2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
265,405 Da
NCBI Official Full Name
neurogenic locus notch homolog protein 2 isoform 1 preproprotein
NCBI Official Synonym Full Names
notch 2
NCBI Official Symbol
NOTCH2
NCBI Official Synonym Symbols
hN2; AGS2; HJCYS
NCBI Protein Information
neurogenic locus notch homolog protein 2; Notch homolog 2
UniProt Protein Name
Neurogenic locus notch homolog protein 2
UniProt Gene Name
NOTCH2
UniProt Synonym Gene Names
Notch 2; hN2
UniProt Entry Name
NOTC2_HUMAN
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Product Notes
The Notch2 notch2 (Catalog #AAA211759) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Notch2 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Notch2 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the Notch2 notch2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Notch2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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