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IF (Immunofluorescence)
(Immunofluorescence of NOX2 in rat brain tissue with NOX2 antibody at 20 μg/ml.Green: NOX2 Antibody (AAA40558)Blue: DAPI staining)
Rabbit NOX2 Polyclonal Antibody | anti-CYBB antibody
NOX2 Antibody
Gene Names
CYBB; CGD; NOX2; IMD34; AMCBX2; GP91-1; GP91PHOX; p91-PHOX; GP91-PHOX
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot, ELISA
Purity
NOX2 antibody is affinity chromatography purified via peptide column.
Synonyms
NOX2, Antibody; NOX2 Antibody; NOX2; CGD; AMCBX2; GP91-1; GP91PHOX; p91-PHOX; GP91-PHOX; Cytochrome b-245 heavy chain; CGD91-phox; Cytochrome b558 subunit beta; cytochrome b-245, beta polypeptide; anti-CYBB antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
NOX2 antibody is human, mouse and rat reactive. At least two isoforms are known to exist; this antibody will detect both isoforms. NOX2 is predicted to not cross-react with other NOX proteins.
Purity/Purification
NOX2 antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
570
Applicable Applications for anti-CYBB antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA
Conjugate
Unconjugated
Immunogen
Rabbit polyclonal NOX2 antibody was raised against a 15 amino acid peptide near the amino terminus of human NOX2.
Buffer
NOX2 antibody is supplied in PBS containing 0.02% sodium azide.
Homology
Predicted species reactivity based on immunogen sequence: Pig: (87%), Bovine: (87%)
Immunogen
Rabbit polyclonal NOX2 antibody was raised against a 15 amino acid peptide near theamino terminus of human NOX2. The immunogen is located within amino acids 140 - 190 of NOX2.
Preparation and Storage
NOX2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
IF (Immunofluorescence)
(Immunofluorescence of NOX2 in rat brain tissue with NOX2 antibody at 20 μg/ml.Green: NOX2 Antibody (AAA40558)Blue: DAPI staining)
IF (Immunofluorescence)
(Immunofluorescence of NOX2 in rat brain tissue with NOX2 antibody at 20 μg/ml.Green: NOX2 Antibody (AAA40558)Blue: DAPI staining)
IHC (Immunohistochemistry)
(Immunohistochemistry of NOX2 in rat brain tissue cells with NOX2 antibody at 2 μg/ml.)
IHC (Immunohistochemistry)
(Immunohistochemistry of NOX2 in rat brain tissue cells with NOX2 antibody at 2 μg/ml.)
IF (Immunofluorescence)
(Immunofluorescence of NOX2 in rat brain tissue with NOX2 antibody at 20 μg/ml.)
IF (Immunofluorescence)
(Immunofluorescence of NOX2 in rat brain tissue with NOX2 antibody at 20 μg/ml.)
IHC (Immunohiostchemistry)
(Immunohistochemistry of NOX2 in rat brain tissue with NOX2 antibody at 5 μg/ml.)
IHC (Immunohiostchemistry)
(Immunohistochemistry of NOX2 in rat brain tissue with NOX2 antibody at 5 μg/ml.)
WB (Western Blot)
(Western blot analysis of NOX2 in rat brain tissue lysate with NOX2 antibody at 1 μg/ml.)
WB (Western Blot)
(Western blot analysis of NOX2 in rat brain tissue lysate with NOX2 antibody at 1 μg/ml.)
Related Product Information for anti-CYBB antibody
The NOX family of NAPDH oxidases is comprised of seven transmembrane proteins that oxidize intracellular NAPDH/NADH, causing electron transport across the membrane and the reduction of molecular oxygen to superoxide (1). NOX2, also known as cytochrome b beta (CYBB) is one of two proteins that make up Cytochrome b-245, thought to be a primary component of the microbicidal oxidase system of phagocytes. NOX2 deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD) (2). Activation of the NOX2 enzyme complex in microglia is thought to be neurotoxic and may play a role in Alzheimer's and Parkinson's disease (3).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted: 59; 63 kDa
Observed: 58 kDa
Observed: 58 kDa
NCBI Official Full Name
cytochrome b-245 heavy chain
NCBI Official Synonym Full Names
cytochrome b-245, beta polypeptide
NCBI Official Symbol
CYBB
NCBI Official Synonym Symbols
CGD; NOX2; IMD34; AMCBX2; GP91-1; GP91PHOX; p91-PHOX; GP91-PHOX
NCBI Protein Information
cytochrome b-245 heavy chain; CGD91-phox; NADPH oxidase 2; p22 phagocyte B-cytochrome; cytochrome b558 subunit beta; neutrophil cytochrome b 91 kDa polypeptide; heme-binding membrane glycoprotein gp91phox; superoxide-generating NADPH oxidase heavy chain subunit
UniProt Protein Name
Cytochrome b-245 heavy chain
UniProt Gene Name
CYBB
UniProt Synonym Gene Names
NOX2; Cytochrome b558 subunit beta
UniProt Entry Name
CY24B_HUMAN
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Product Notes
The CYBB cybb (Catalog #AAA40558) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NOX2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NOX2 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the CYBB cybb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NOX2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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