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product-image-AAA11515_WB3.jpg WB (Western Blot) (Applications: Western BlotSample: 22RV1 cytosolic and membrane fractionsDetection: Enhanced chemiluminescence"We used this antibody to study the inhibitory effect of GPE on hypoxia induced translocation of gp91phox subunit from cytosol to membrane in our recently published paper (Scientific Reports 6, Article number: 23135 (2016); doi:10.1038/srep23135). This antibody is very much specific in our in vitro system.")

Rabbit NOX2/gp91phox Polyclonal Antibody | anti-CYBB antibody

Anti-NOX2/gp91phox antibody

Gene Names
CYBB; CGD; NOX2; IMD34; AMCBX2; GP91-1; GP91PHOX; p91-PHOX; GP91-PHOX
Reactivity
Reacts with Human , Rat
Predicted to work with: Mouse
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified.
Synonyms
NOX2/gp91phox, Antibody; Anti-NOX2/gp91phox antibody; Cytochrome b-245 heavy chain; cytochrome b-245, beta polypeptide; AMCBX2 antibody; CGD antibody; CGD91-phox antibody; CY24B_HUMAN antibody; CYBB antibody; Cytochrome b 245, beta polypeptide antibody; Cytochrome b(558) beta chain antibody; Cytochrome b(558) subunit beta antibody; Cytochrome b-245 heavy chain antibody; Cytochrome b558 subunit beta antibody; GP91 PHOX antibody; gp91-1 antibody; gp91-phox antibody; GP91PHOX antibody; Heme-binding membrane glycoprotein gp91phox antibody; NADPH oxidase 2 antibody; Neutrophil cytochrome b 91 kDa polypeptide antibody; NOX2 antibody; p22 phagocyte B-cytochrome antibody; P91 PHOX antibody; p91-PHOX antibody; Superoxide-generating NADPH oxidase heavy chain subunit antibody; anti-CYBB antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Reacts with Human , Rat
Predicted to work with: Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
No cross reactivity with other proteins
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Sequence Length
570
Applicable Applications for anti-CYBB antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes
Western Blot: Concentration: 0.1-0.5 ug/ml, Tested Species: Human, Rat, Predicted Species: Mouse
Immunohistochemistry (Paraffin-embedded Section): Concentration: 0.5-1 ug/ml, Tested Species: Human, Rat, Predicted Species: Mouse, Antigen Retrieval: By Heat

*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.

Tested Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.

Other applications have not been tested.
Optimal dilutions should be determined by end users.
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human NOX2 (442-459aa YWLCRDTHAFEWFADLLQ), identical to the related rat and mouse sequences.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500 ug/ml.
Relevant Detection Systems
The lab provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit in Western Blot, supported by in IHC(P).
Preparation and Storage
At -20°C. After reconstitution, at 4°C. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

WB (Western Blot)

(Applications: Western BlotSample: 22RV1 cytosolic and membrane fractionsDetection: Enhanced chemiluminescence"We used this antibody to study the inhibitory effect of GPE on hypoxia induced translocation of gp91phox subunit from cytosol to membrane in our recently published paper (Scientific Reports 6, Article number: 23135 (2016); doi:10.1038/srep23135). This antibody is very much specific in our in vitro system.")

product-image-AAA11515_WB3.jpg WB (Western Blot) (Applications: Western BlotSample: 22RV1 cytosolic and membrane fractionsDetection: Enhanced chemiluminescence"We used this antibody to study the inhibitory effect of GPE on hypoxia induced translocation of gp91phox subunit from cytosol to membrane in our recently published paper (Scientific Reports 6, Article number: 23135 (2016); doi:10.1038/srep23135). This antibody is very much specific in our in vitro system.")

IHC (Immunohistochemistry)

(Anti-NOX2/gp91phox antibody, AAA11515, IHC(P)IHC(P): Human Intestinal Cancer Tissue)

product-image-AAA11515_IHC2.jpg IHC (Immunohistochemistry) (Anti-NOX2/gp91phox antibody, AAA11515, IHC(P)IHC(P): Human Intestinal Cancer Tissue)

WB (Western Blot)

(Anti-NOX2/gp91phox antibody, AAA11515, Western blottingLane 1: HELA Cell LysateLane 2: JURKAT Cell LysateLane 3: MCF-7 Cell LysateLane 4: SMMC Cell Lysate)

product-image-AAA11515_WB.jpg WB (Western Blot) (Anti-NOX2/gp91phox antibody, AAA11515, Western blottingLane 1: HELA Cell LysateLane 2: JURKAT Cell LysateLane 3: MCF-7 Cell LysateLane 4: SMMC Cell Lysate)
Related Product Information for anti-CYBB antibody
Background: NOX2(NADPH OXIDASE 2), also called CYBB(CYTOCHROME b(-245), BETA SUBUNIT), p91-PHOX or GP91-1, is a human gene encoding a glycoprotein.NOX2 is an essential component of phagocytic NADPH-oxidase, a membrane-bound enzyme complex that generates large quantities of microbicidal superoxide and other oxidants upon activation. It is mapped on Xp11.4. NOX2 is a heterodimer composed of an alpha chain of relative molecular mass 23 kD and a beta chain of 76 to 82 kD. NOX2 assembled on DC phagosomes in a gp91-phox subunit-dependent manner, and that reactive oxygen species were produced in a more sustained manner in immature DC phagosomes than in macrophage phagosomes. As a major player in innate immune responses in neutrophils, NOX2 is also involved in adaptive immunity through its activity in DCs. In heart cells, physiologic stretch rapidly activates reduced-form NOX2 to produce reactive oxygen species(ROS) in a process dependent on microtubules(X-ROS signaling).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Cytochrome b-245 heavy chain
NCBI Official Synonym Full Names
cytochrome b-245, beta polypeptide
NCBI Official Symbol
CYBB
NCBI Official Synonym Symbols
CGD; NOX2; IMD34; AMCBX2; GP91-1; GP91PHOX; p91-PHOX; GP91-PHOX
NCBI Protein Information
cytochrome b-245 heavy chain; CGD91-phox; NADPH oxidase 2; p22 phagocyte B-cytochrome; cytochrome b558 subunit beta; neutrophil cytochrome b 91 kDa polypeptide; heme-binding membrane glycoprotein gp91phox; superoxide-generating NADPH oxidase heavy chain subunit
UniProt Protein Name
Cytochrome b-245 heavy chain
UniProt Gene Name
CYBB
UniProt Synonym Gene Names
NOX2; Cytochrome b558 subunit beta
UniProt Entry Name
CY24B_HUMAN

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Product Notes

The CYBB cybb (Catalog #AAA11515) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-NOX2/gp91phox antibody reacts with Reacts with Human, Rat Predicted to work with: Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NOX2/gp91phox can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin. Western Blot: Concentration: 0.1-0.5 ug/ml, Tested Species: Human, Rat, Predicted Species: Mouse Immunohistochemistry (Paraffin-embedded Section): Concentration: 0.5-1 ug/ml, Tested Species: Human, Rat, Predicted Species: Mouse, Antigen Retrieval: By Heat *The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Researchers should empirically determine the suitability of the CYBB cybb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NOX2/gp91phox, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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