IHC (Immunohistochemisry)
(NPC1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NPC1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Rabbit anti-Human NPC1 Polyclonal Antibody | anti-NPC1 antibody
NPC1 Antibody (Center)
Gene Names
NPC1; NPC
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, ELISA, Immunofluorescence
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
NPC1, Antibody; NPC1 Antibody (Center); Niemann-Pick C1 protein; NPC1; anti-NPC1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This NPC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 591-620 amino acids from the Central region of human NPC1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.35 (varies by lot)
Sequence Positions
591-620
Sequence Length
960
Applicable Applications for anti-NPC1 antibody
WB (Western Blot), IHC (Immunohistochemistry), ELISA, IF (Immunofluorescence)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistochemisry)
(NPC1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NPC1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohistochemisry)
(NPC1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NPC1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of NPC1 Antibody (Center) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of NPC1 Antibody (Center) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
WB (Western Blot)
(NPC1 Antibody (Center) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the NPC1 antibody detected the NPC1 protein (arrow).)
WB (Western Blot)
(NPC1 Antibody (Center) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the NPC1 antibody detected the NPC1 protein (arrow).)
Related Product Information for anti-NPC1 antibody
This gene encodes a large protein that resides in the
limiting membrane of endosomes and lysosomes and mediates
intracellular cholesterol trafficking via binding of cholesterol to
its N-terminal domain. It is predicted to have a cytoplasmic
C-terminus, 13 transmembrane domains, and 3 large loops in the
lumen of the endosome - the last loop being at the N-terminus. This
protein transports low-density lipoproteins to late
endosomal/lysosomal compartments where they are hydrolized and
released as free cholesterol. Defects in this gene cause
Niemann-Pick type C disease, a rare autosomal recessive
neurodegenerative disorder characterized by over accumulation of
cholesterol and glycosphingolipids in late endosomal/lysosomal
compartments.
limiting membrane of endosomes and lysosomes and mediates
intracellular cholesterol trafficking via binding of cholesterol to
its N-terminal domain. It is predicted to have a cytoplasmic
C-terminus, 13 transmembrane domains, and 3 large loops in the
lumen of the endosome - the last loop being at the N-terminus. This
protein transports low-density lipoproteins to late
endosomal/lysosomal compartments where they are hydrolized and
released as free cholesterol. Defects in this gene cause
Niemann-Pick type C disease, a rare autosomal recessive
neurodegenerative disorder characterized by over accumulation of
cholesterol and glycosphingolipids in late endosomal/lysosomal
compartments.
Product Categories/Family for anti-NPC1 antibody
References
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Fontaine-Bisson, B., et al. Diabetologia 53(10):2155-2162(2010)
Kagedal, K., et al. Biochim. Biophys. Acta 1801(8):831-838(2010)
Rodriguez-Rodriguez, E., et al. J. Alzheimers Dis. 21(2):619-625(2010)
Ma, W., et al. BMC Med. Genet. 11, 149 (2010) :
Fontaine-Bisson, B., et al. Diabetologia 53(10):2155-2162(2010)
Kagedal, K., et al. Biochim. Biophys. Acta 1801(8):831-838(2010)
Rodriguez-Rodriguez, E., et al. J. Alzheimers Dis. 21(2):619-625(2010)
Ma, W., et al. BMC Med. Genet. 11, 149 (2010) :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
142167
NCBI Official Full Name
Niemann-Pick C1 protein
NCBI Official Synonym Full Names
Niemann-Pick disease, type C1
NCBI Official Symbol
NPC1
NCBI Official Synonym Symbols
NPC
NCBI Protein Information
Niemann-Pick C1 protein
UniProt Protein Name
Niemann-Pick C1 protein
UniProt Gene Name
NPC1
UniProt Entry Name
NPC1_HUMAN
Similar Products
Product Notes
The NPC1 npc1 (Catalog #AAA284344) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 591-620. The NPC1 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NPC1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), ELISA, IF (Immunofluorescence). Researchers should empirically determine the suitability of the NPC1 npc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NPC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.