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FCM/FACS (Flow Cytometry)
(NPM1 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit NPM1 Polyclonal Antibody | anti-NPM1 antibody
NPM1 Antibody (N-term)
Gene Names
NPM1; B23; NPM
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Applications
Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
NPM1, Antibody; NPM1 Antibody (N-term); Nucleophosmin; NPM; Nucleolar phosphoprotein B23; Nucleolar protein NO38; Numatrin; NPM1; anti-NPM1 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This NPM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 7-33 amino acids from the N-terminal region of human NPM1.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
7-33
Sequence Length
259
Applicable Applications for anti-NPM1 antibody
WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(NPM1 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(NPM1 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human lymph tissue reacted with NPM1 antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human lymph tissue reacted with NPM1 antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IF (Immunofluorescence)
(Fluorescent image of A549 cell stained with NPM1 Antibody (N-term). A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NPM1 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree).NPM1 immunoreactivity is localized to Nucleus and Nucleolus significantly.)
IF (Immunofluorescence)
(Fluorescent image of A549 cell stained with NPM1 Antibody (N-term). A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NPM1 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree).NPM1 immunoreactivity is localized to Nucleus and Nucleolus significantly.)
WB (Western Blot)
(NPM1 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the NPM1 antibody detected the NPM1 protein (arrow).)
WB (Western Blot)
(NPM1 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the NPM1 antibody detected the NPM1 protein (arrow).)
Related Product Information for anti-NPM1 antibody
NPM1 is a ubiquitously expressed nucleolar protein that shuttles between the nucleus and cytoplasm. It is implicated in multiple functions, including ribosomal protein assembly and transport, control of centrosome duplication, and regulation of the tumor suppressor ARF. NPM1 mutations that relocalize NPM1 from the nucleus into the cytoplasm are associated with development of acute myeloid leukemia.
Product Categories/Family for anti-NPM1 antibody
References
Vascotto,C., Mol. Cell. Biol. 29 (7), 1834-1854 (2009)
Ma,W., Cancer Biomark 5 (1), 51-58 (2009)
Zhang,H., J. Biol. Chem. 279 (34), 35726-35734 (2004)
Ma,W., Cancer Biomark 5 (1), 51-58 (2009)
Zhang,H., J. Biol. Chem. 279 (34), 35726-35734 (2004)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
32575
NCBI Official Full Name
nucleophosmin isoform 3
NCBI Official Synonym Full Names
nucleophosmin (nucleolar phosphoprotein B23, numatrin)
NCBI Official Symbol
NPM1
NCBI Official Synonym Symbols
B23; NPM
NCBI Protein Information
nucleophosmin
UniProt Protein Name
Nucleophosmin
UniProt Gene Name
NPM1
UniProt Synonym Gene Names
NPM; NPM
UniProt Entry Name
NPM_HUMAN
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Product Notes
The NPM1 npm1 (Catalog #AAA284392) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 7-33. The NPM1 Antibody (N-term) reacts with Human (Predicted Reactivity: Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's NPM1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the NPM1 npm1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NPM1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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