Rabbit NR5A1 Polyclonal Antibody | anti-NR5A1 antibody

NR5A1 Antibody (N-term)

Cat. Num. AAA285979

• Gene Names: NR5A1; ELP; SF1; FTZ1; POF7; SF-1; AD4BP; FTZF1; SPGF8; SRXY3
• Reactivity: Human; Predicted Reactivity: Bovine, Mouse, Pig, Rat
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: NR5A1, Antibody; NR5A1 Antibody (N-term); Steroidogenic factor 1; SF-1; STF-1; Adrenal 4-binding protein; Fushi tarazu factor homolog 1; Nuclear receptor subfamily 5 group A member 1; Steroid hormone receptor Ad4BP; NR5A1; AD4BP; FTZF1; SF1; anti-NR5A1 antibody

• Host: Rabbit
• Reactivity: Human; Predicted Reactivity: Bovine, Mouse, Pig, Rat
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This NR5A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 64-93 amino acids from the N-terminal region of human NR5A1.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Positions: 64-93
• Sequence Length: 461

• Applicable Applications for anti-NR5A1 antibody: FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
• Antigen Type: Synthetic Peptide
• Tissue Location: Tissue Location
• Cellular Location: Nucleus - {ECO:0000255|PROSITE-ProRule:PRU00407, ECO:0000269|PubMed:11479297, ECO:0000269|PubMed:27490115}
• Antigen Source: HUMAN
• Function: Transcriptional activator. Essential for sexual differentiation and formation of the primary steroidogenic tissues (PubMed: ). Binds to the Ad4 site found in the promoter region of steroidogenic P450 genes such as CYP11A, CYP11B and CYP21B. Also regulates the AMH/Muellerian inhibiting substance gene as well as the AHCH and STAR genes. 5'-YCAAGGYC-3' and 5'-RRAGGTCA-3' are the consensus sequences for the recognition by NR5A1 (PubMed: ). The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional activity. Binds phosphatidylcholine (By similarity). Binds phospholipids with a phosphatidylinositol (PI) headgroup, in particular PI(3,4)P2 and PI(3,4,5)P3. Activated by the phosphorylation of NR5A1 by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation.
• Preparation and Storage: Storage Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots. Avoid repeated freeze/thaw cycles.

FCM/FACS (Flow Cytometry)

(NR5A1 Antibody (N-term) (AAA285979) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohiostchemistry)

(NR5A1 Antibody (N-term) (AAA285979) IHC analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NR5A1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot)

(Western blot analysis of NR5A1 Antibody (N-term) (AAA285979) in HepG2 cell line lysates (35ug/lane).NR5A1 (arrow) was detected using the purified Pab.)

Related Product Information for anti-NR5A1 antibody

NR5A1 is a transcriptional activator involved in sex determination. The encoded protein binds DNA as a monomer. Defects in this protein are a cause of XY sex reversal with or without adrenal failure as well as adrenocortical insufficiency without ovarian defect.

Product Categories/Family for anti-NR5A1 antibody

Developmental Biology

References

Almeida, M.Q., et al. J. Clin. Endocrinol. Metab. 95(3):1458-1462(2010)
Ramayya, M.S., et al. J. Steroid Biochem. Mol. Biol. 119 (1-2), 14-25 (2010)
Catalano, S., et al. J. Biol. Chem. 285(8):5581-5593(2010)

NCBI and Uniprot Product Information

• NCBI GI #: 20070193
• NCBI GeneID: 2516
• NCBI Accession #: NP_004950.2
• NCBI GenBank Nucleotide #: NM_004959.4
• UniProt Accession #: Q13285; O15196; Q5T6F5
• Molecular Weight: 51636
• NCBI Official Full Name: steroidogenic factor 1
• NCBI Official Synonym Full Names: nuclear receptor subfamily 5, group A, member 1
• NCBI Official Symbol: NR5A1
• NCBI Official Synonym Symbols: ELP; SF1; FTZ1; POF7; SF-1; AD4BP; FTZF1; SPGF8; SRXY3
• NCBI Protein Information: steroidogenic factor 1
• UniProt Protein Name: Steroidogenic factor 1
• UniProt Gene Name: NR5A1
• UniProt Synonym Gene Names: AD4BP; FTZF1; SF1; SF-1; STF-1
• UniProt Entry Name: STF1_HUMAN