WB (Western Blot)
(Detection of human NUMA by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NUMA antibody AAA211509 used for WB at 0.04 ug/ml (A) and 0.1 ug/ml (B) and used for IP at 3 ug/mg lysate. NUMA was also immunoprecipitated by rabbit anti-NUMA antibody which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 seconds (A and B).)
Rabbit anti-Human NUMA Polyclonal Antibody | anti-NUMA antibody
Rabbit anti-NUMA Antibody, Affinity Purified
Gene Names
NUMA1; NUMA
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
NUMA, Antibody; Rabbit anti-NUMA Antibody, Affinity Purified; NUMA; nuclear mitotic apparatus protein 1; NuMA protein; structural nuclear protein; nuclear matrix protein-22; NMP-22; centrophilin stabilizes mitotic spindle in mitotic cells; SP-H antigen; Nuclear mitotic apparatus protein; Nuclear mitotic apparatus protein 1; anti-NUMA antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-NUMA antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 925 and 975
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human NUMA by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NUMA antibody AAA211509 used for WB at 0.04 ug/ml (A) and 0.1 ug/ml (B) and used for IP at 3 ug/mg lysate. NUMA was also immunoprecipitated by rabbit anti-NUMA antibody which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 seconds (A and B).)
WB (Western Blot)
(Detection of human NUMA by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NUMA antibody AAA211509 used for WB at 0.04 ug/ml (A) and 0.1 ug/ml (B) and used for IP at 3 ug/mg lysate. NUMA was also immunoprecipitated by rabbit anti-NUMA antibody which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 seconds (A and B).)
IHC (Immunohistochemistry)
(Detection of human NUMA by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-NUMA (Cat. No. AAA211509 Lot1) used at a dilution of 1:400 (0.5ug/ml). Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight 594 Conjugated used at a dilution of 1:100.)
IHC (Immunohistochemistry)
(Detection of human NUMA by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-NUMA (Cat. No. AAA211509 Lot1) used at a dilution of 1:400 (0.5ug/ml). Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight 594 Conjugated used at a dilution of 1:100.)
Related Product Information for anti-NUMA antibody
The nuclear mitotic apparatus protein (NuMA) is a critical component of the mitotic spindle that is responsible for organizing minus-ends of microtubules at spindle poles. NUMA is regulated by phosphorylation and associates with dynein and dynactin in its phosphorylated form. Dephosphorylation of NUMA in anaphase results in the release from dynein and dynactin to allow spindle disassembly. NUMA, in association with dynein and dynactin, also interacts with 4.1R in a complex of spindle pole organizing proteins. Other NUMA interacting proteins include GAS41, tankyrase, and Rae1. These interactions indicate a broader role for NUMA function in the nucleus.
Product Categories/Family for anti-NUMA antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
238,260 Da
NCBI Official Full Name
nuclear mitotic apparatus protein 1
NCBI Official Synonym Full Names
nuclear mitotic apparatus protein 1
NCBI Official Symbol
NUMA1
NCBI Official Synonym Symbols
NUMA
NCBI Protein Information
nuclear mitotic apparatus protein 1; SP-H antigen; structural nuclear protein; centrophilin stabilizes mitotic spindle in mitotic cells
UniProt Protein Name
Nuclear mitotic apparatus protein 1
UniProt Gene Name
NUMA1
UniProt Synonym Gene Names
NUMA; NuMA protein
UniProt Entry Name
NUMA1_HUMAN
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Product Notes
The NUMA numa1 (Catalog #AAA211509) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-NUMA Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NUMA can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the NUMA numa1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NUMA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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