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product-image-AAA289580_FCM13.jpg FCM/FACS (Flow Cytometry) (NUP153 Antibody (N-term) flow cytometry analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Rabbit anti-Human NUP153 Polyclonal Antibody | anti-NUP153 antibody

NUP153 Antibody (N-term)

Gene Names
NUP153; N153; HNUP153
Reactivity
Human
Applications
Western Blot, ELISA, Flow Cytometry, Functional Assay
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
NUP153, Antibody; NUP153 Antibody (N-term); Nuclear pore complex protein Nup153; 153 kDa nucleoporin; Nucleoporin Nup153; NUP153; anti-NUP153 antibody
Ordering
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This NUP153 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 58-86 amino acids from the N-terminal region of human NUP153.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
58-86
Sequence Length
1,506
Applicable Applications for anti-NUP153 antibody
WB (Western Blot), ELISA, FCM/FACS (Flow Cytometry)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

FCM/FACS (Flow Cytometry)

(NUP153 Antibody (N-term) flow cytometry analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

product-image-AAA289580_FCM13.jpg FCM/FACS (Flow Cytometry) (NUP153 Antibody (N-term) flow cytometry analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

WB (Western Blot)

(Western blot analysis of NUP153 Antibody (N-term) in Hela cell line lysates (35ug/lane). NUP153 (arrow) was detected using the purified Pab.)

product-image-AAA289580_WB15.jpg WB (Western Blot) (Western blot analysis of NUP153 Antibody (N-term) in Hela cell line lysates (35ug/lane). NUP153 (arrow) was detected using the purified Pab.)
Related Product Information for anti-NUP153 antibody
Nuclear pore complexes are extremely elaborate structures that mediate the regulated movement of macromolecules between the nucleus and cytoplasm. These complexes are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. Nucleoporins are pore complex-specific glycoproteins characterized by cytoplasmically oriented O-linked N-acetylglucosamine residues and numerous repeats of the pentapeptide sequence XFXFG. This protein has three distinct domains: a N-terminal region within which a pore targeting domain has been identified, a central region containing multiple zinc finger motifs, and a C-terminal region containing multiple XFXFG repeats.
Product Categories/Family for anti-NUP153 antibody
References
Olsen,J.V., et.al., Cell 127 (3), 635-648 (2006)
Ullman,K.S.,et.al., Mol. Biol. Cell 10 (3), 649-664 (1999)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
153938
NCBI Official Full Name
nuclear pore complex protein Nup153 isoform 2
NCBI Official Synonym Full Names
nucleoporin 153kDa
NCBI Official Symbol
NUP153
NCBI Official Synonym Symbols
N153; HNUP153
NCBI Protein Information
nuclear pore complex protein Nup153
UniProt Protein Name
Nuclear pore complex protein Nup153
UniProt Gene Name
NUP153
UniProt Entry Name
NU153_HUMAN

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Product Notes

The NUP153 nup153 (Catalog #AAA289580) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 58-86. The NUP153 Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NUP153 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the NUP153 nup153 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NUP153, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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