WB (Western Blot)
(Anti-OLIG1 rabbit polyclonal antibody at 1:500 dilutionLane A: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:27 kDaObserved band size:35 kDa)
Rabbit anti-Human Olig1 Polyclonal Antibody | anti-OLIG1 antibody
Anti-Olig1 Antibody, Rabbit Polyclonal
Gene Names
OLIG1; BHLHB6; BHLHE21
Reactivity
Human
Applications
Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
Olig1, Antibody; Anti-Olig1 Antibody, Rabbit Polyclonal; OLIG1 Antibody, Rabbit PAb, Antigen Affinity Purified; oligodendrocyte transcription factor 1; Anti-BHLHB6 Antibody; Anti-BHLHE21 Antibody; anti-OLIG1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human OLIG1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-OLIG1 antibody
IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to the center region of the human OLIG1
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the human OLIG1, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
WB (Western Blot)
(Anti-OLIG1 rabbit polyclonal antibody at 1:500 dilutionLane A: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:27 kDaObserved band size:35 kDa)
WB (Western Blot)
(Anti-OLIG1 rabbit polyclonal antibody at 1:500 dilutionLane A: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:27 kDaObserved band size:35 kDa)
IP (Immunoprecipitation)
(OLIG1 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate0.5 uL anti-OLIG1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-OLIG1 rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 20 kDaObserved band size: 20 kDa)
IP (Immunoprecipitation)
(OLIG1 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate0.5 uL anti-OLIG1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-OLIG1 rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 20 kDaObserved band size: 20 kDa)
IF (Immunofluorescence)
(Immunofluorescence staining of OLIG1 in SHSY5Y cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human OLIG1 polyclonal antibody (1:5000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor594-conjugated Goat Anti-rabbit IgG secondary antibody (red)Positive staining was localized to nucleus.)
IF (Immunofluorescence)
(Immunofluorescence staining of OLIG1 in SHSY5Y cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human OLIG1 polyclonal antibody (1:5000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor594-conjugated Goat Anti-rabbit IgG secondary antibody (red)Positive staining was localized to nucleus.)
Related Product Information for anti-OLIG1 antibody
OLIG1 is an oligodendrocyte (OL) transcription factor, which can contribute to the proliferation and differentiation of OLs, and the maturation of myelin.OLIG1 and OLIG2 are basic-helix-loop-helix (bHLH) transcription factors expressed in the pMN domain of the spinal cord, which sequentially generates motoneurons and oligodendrocytes. Multivariate analyses of our data confirmed that OLIG1 protein expression significantly correlates with overall survival in NSCLC patients, with a relative risk of 0.84 (95% confidence interval 0.77-0.91, p <0.001) along with T and N stages, as indicated by a Cox proportional hazard model. So OLIG1 protein expression could be utilized as a novel prognostic factor, which could aid in deciding which NSCLC patients might benefit from more aggressive therapy.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted: 30 kDa
Observed: 29 kDa
Observed: 29 kDa
NCBI Official Full Name
oligodendrocyte transcription factor 1
NCBI Official Synonym Full Names
oligodendrocyte transcription factor 1
NCBI Official Symbol
OLIG1
NCBI Official Synonym Symbols
BHLHB6; BHLHE21
NCBI Protein Information
oligodendrocyte transcription factor 1; oligo1; class B basic helix-loop-helix protein 6; class E basic helix-loop-helix protein 21; oligodendrocyte lineage transcription factor 1; basic domain, helix-loop-helix protein, class B, 6; oligodendrocyte-specif
UniProt Protein Name
Oligodendrocyte transcription factor 1
UniProt Gene Name
OLIG1
UniProt Synonym Gene Names
BHLHB6; BHLHE21; Oligo1; bHLHb6; bHLHe21
UniProt Entry Name
OLIG1_HUMAN
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Product Notes
The OLIG1 olig1 (Catalog #AAA254282) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Olig1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Olig1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the OLIG1 olig1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Olig1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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