IHC (Immunohiostchemistry)
(Anti- Oncostatin M Picoband antibody, AAA46343, IHC(P)IHC(P): Human Intestinal Cancer Tissue)
anti-Human Oncostatin M Polyclonal Antibody | anti-OSM antibody
Anti-Oncostatin M Antibody
Reactivity
Human
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Immunogen Affinity Purified
Synonyms
Oncostatin M, Antibody; Anti-Oncostatin M Antibody; Oncostatin-M; MGC20461; ONCM_HUMAN; Oncostatin M; Oncostatin M precursor; OSM antibody; oncostatin M; anti-OSM antibody
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Immunogen Affinity Purified
Form/Format
Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
231
Applicable Applications for anti-OSM antibody
ELISA, IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
E Coli-derived human Oncostatin M recombinant protein (Position: A26-R220). Human Oncostatin M shares 47.1% and 47.6% amino acid (aa) sequence identity with mouse and rat Oncostatin M, respectively.
Ig Type
Rabbit IgG
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
IHC (Immunohiostchemistry)
(Anti- Oncostatin M Picoband antibody, AAA46343, IHC(P)IHC(P): Human Intestinal Cancer Tissue)
IHC (Immunohiostchemistry)
(Anti- Oncostatin M Picoband antibody, AAA46343, IHC(P)IHC(P): Human Intestinal Cancer Tissue)
WB (Western Blot)
(Anti- Oncostatin M Picoband antibody, AAA46343, Western blottingAll lanes: Anti Oncostatin M (AAA46343) at 0.5ug/mlLane 1: A549 Whole Cell Lysate at 40ugLane 2: HUT Whole Cell Lysate at 40ugLane 3: JURKAT Whole Cell Lysate at 40ugLane 4: SW620 Whole Cell Lysate at 40ugLane 5: MCF-7 Whole Cell Lysate at 40ugPredicted bind size: 28KDObserved bind size: 40KD)
WB (Western Blot)
(Anti- Oncostatin M Picoband antibody, AAA46343, Western blottingAll lanes: Anti Oncostatin M (AAA46343) at 0.5ug/mlLane 1: A549 Whole Cell Lysate at 40ugLane 2: HUT Whole Cell Lysate at 40ugLane 3: JURKAT Whole Cell Lysate at 40ugLane 4: SW620 Whole Cell Lysate at 40ugLane 5: MCF-7 Whole Cell Lysate at 40ugPredicted bind size: 28KDObserved bind size: 40KD)
Related Product Information for anti-OSM antibody
Description: Rabbit IgG polyclonal antibody for Oncostatin-M(OSM) detection. Tested with WB, IHC-P, ELISA in Human.
Background: OSM (ONCOSTATIN M) is a member of a cytokine family that includes leukemia-inhibitory factor, granulocyte colony-stimulating factor, and interleukin 6. This gene encodes a growth regulator which inhibits the proliferation of a number of tumor cell lines. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. OSM is mapped on 22q12.2. It has the ability to inhibit the growth of human A375 melanoma cells but not normal human fibroblasts. Treatment with recombinant OSM leads to the inhibition of proliferation and changes in cellular morphology of a number of tumor cell lines derived from a wide variety of tissue types. OSM also has the ability to inhibit the proliferation of murine M1 myeloid leukemic cells and can induce their differentiation into macrophage-like cells, a function shared by LIF, CSF3, and IL6. The direction of gene transcription was telomeric to centromeric, with the OSM gene located upstream of the LIF gene.
Background: OSM (ONCOSTATIN M) is a member of a cytokine family that includes leukemia-inhibitory factor, granulocyte colony-stimulating factor, and interleukin 6. This gene encodes a growth regulator which inhibits the proliferation of a number of tumor cell lines. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. OSM is mapped on 22q12.2. It has the ability to inhibit the growth of human A375 melanoma cells but not normal human fibroblasts. Treatment with recombinant OSM leads to the inhibition of proliferation and changes in cellular morphology of a number of tumor cell lines derived from a wide variety of tissue types. OSM also has the ability to inhibit the proliferation of murine M1 myeloid leukemic cells and can induce their differentiation into macrophage-like cells, a function shared by LIF, CSF3, and IL6. The direction of gene transcription was telomeric to centromeric, with the OSM gene located upstream of the LIF gene.
References
1. Miles, S. A., Martinez-Maza, O., Rezai, A., Magpantay, L., Kishimoto, T., Nakamura, S., Radka, S. F., Linsley, P. S. Oncostatin M as a potent mitogen for AIDS-Kaposi's sarcoma-derived cells. Science 255: 1432-1434, 1992. 2. Modur, V., Feldhaus, M. J., Weyrich, A. S., Jicha, D. L., Prescott, S. M., Zimmerman, G. A., McIntyre, T. M. Oncostatin M is a proinflammatory mediator: in vivo effects correlate with endothelial cell expression of inflammatory cytokines and adhesion molecules. J. Clin. Invest. 100: 158-168, 1997. 3. Rose, T. M., Bruce, A. G. Oncostatin M is a member of a cytokine family that includes leukemia-inhibitory factor, granulocyte colony-stimulating factor, and interleukin 6. Proc. Nat. Acad. Sci. 88: 8641-8645, 1991.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28,484 Da
NCBI Official Full Name
oncostatin-M isoform 2
NCBI Official Synonym Full Names
oncostatin M
NCBI Official Symbol
OSM
NCBI Protein Information
oncostatin-M
UniProt Protein Name
Oncostatin-M
UniProt Gene Name
OSM
UniProt Synonym Gene Names
OSM
UniProt Entry Name
ONCM_HUMAN
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Product Notes
The OSM osm (Catalog #AAA46343) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-Oncostatin M Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Oncostatin M can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the OSM osm for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Oncostatin M, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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