Application Data
(Formalin fixed, paraffin embedded human spleen stained with Rabbit anti Human ORAI1 (AAA50018) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0))
Rabbit anti-Mouse, Rat ORAI1 Polyclonal Antibody | anti-ORAI1 antibody
RABBIT ANTI ORAI1 (C-TERMINAL)
Gene Names
ORAI1; IMD9; TAM2; ORAT1; CRACM1; TMEM142A
Reactivity
Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Synonyms
ORAI1, Antibody; RABBIT ANTI ORAI1 (C-TERMINAL); anti-ORAI1 antibody
Host
Rabbit
Reactivity
Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
This item recognises human ORAI1, also known as Transmembrane protein 142A (TMEM142A), a 32.7 kDa multi-pass membrane protein belonging to the ORAI family.
Antigen stimulation of immune cells triggers Ca2+ entry through Ca2+ release-activated Ca2+ (CRAC) channels. CRAC channels are the main pathway for Ca2+ influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT.
ORAI1 is a four- transmembrane spanning protein that is an essential component of CRAC. ORAI1 functions as a highly selective Ca2+ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca2+ sensor.
Defective ORAI1 in humans is the cause of one form of hereditary severe combined immune deficiency (SCID), which results in ablated T-cell Ca2+ entry.
This item is not expected to cross react with either ORAI2 or ORAI3.
Antigen stimulation of immune cells triggers Ca2+ entry through Ca2+ release-activated Ca2+ (CRAC) channels. CRAC channels are the main pathway for Ca2+ influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT.
ORAI1 is a four- transmembrane spanning protein that is an essential component of CRAC. ORAI1 functions as a highly selective Ca2+ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca2+ sensor.
Defective ORAI1 in humans is the cause of one form of hereditary severe combined immune deficiency (SCID), which results in ablated T-cell Ca2+ entry.
This item is not expected to cross react with either ORAI2 or ORAI3.
Form/Format
Purified
Purified IgG - liquid
Purified IgG - liquid
Concentration
IgG concentration 1.0mg/ml (varies by lot)
Sequence Length
301
Applicable Applications for anti-ORAI1 antibody
WB (Western Blot), IHC (Immunohistochemistry)
Perservative Stabilisers
0.02% Sodium Azide (NaN3)
Immunogen
A 16 amino acid peptide from near the carboxy terminus of human ORAI1.
Buffer Solution
Buffer Solution
Antiserum Preparation
Antiserum to human ORAI1 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Target Species
Human
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Application Data
(Formalin fixed, paraffin embedded human spleen stained with Rabbit anti Human ORAI1 (AAA50018) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0))
Application Data
(Formalin fixed, paraffin embedded human spleen stained with Rabbit anti Human ORAI1 (AAA50018) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0))
Application Data
(Western blot analysis of human ovary probed with Rabbit anti Human ORAI1 (AAA50018) at 0.5(A), 1(B) and 2(C) ug/ml)
Application Data
(Western blot analysis of human ovary probed with Rabbit anti Human ORAI1 (AAA50018) at 0.5(A), 1(B) and 2(C) ug/ml)
Related Product Information for anti-ORAI1 antibody
MBS222945 recognises human ORAI1, also known as Transmembrane protein 142A (TMEM142A), a 32.7 kDa multi-pass membrane protein belonging to the ORAI family. Antigen stimulation of immune cells triggers Ca2+ entry through Ca2+ release-activated Ca2+ (CRAC) channels. CRAC channels are the main pathway for Ca2+ influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT. ORAI1 is a four- transmembrane spanning protein that is an essential component of CRAC. ORAI1 functions as a highly selective Ca2+ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca2+ sensor. Defective ORAI1 in humans is the cause of one form of hereditary severe combined immune deficiency (SCID), which results in ablated T-cell Ca2+ entry. MBS222945 is not expected to cross react with either ORAI2 or ORAI3.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25,337 Da
NCBI Official Full Name
calcium release-activated calcium channel protein 1
NCBI Official Synonym Full Names
ORAI calcium release-activated calcium modulator 1
NCBI Official Symbol
ORAI1
NCBI Official Synonym Symbols
IMD9; TAM2; ORAT1; CRACM1; TMEM142A
NCBI Protein Information
calcium release-activated calcium channel protein 1; calcium release-activated calcium modulator 1; protein orai-1; transmembrane protein 142A
UniProt Protein Name
Calcium release-activated calcium channel protein 1
UniProt Gene Name
ORAI1
UniProt Synonym Gene Names
CRACM1; TMEM142A
UniProt Entry Name
CRCM1_HUMAN
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Product Notes
The ORAI1 orai1 (Catalog #AAA50018) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RABBIT ANTI ORAI1 (C-TERMINAL) reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ORAI1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the ORAI1 orai1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ORAI1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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