WB (Western Blot)
(Detection of human ORC2 by western blot. Samples: Whole cell lysate from HeLa (5, 15, and 50 ug) and HEK293T (T; 50 ug) cells. Antibody: Affinity purified rabbit anti-ORC2 antibody AAA211991 (lot AAA211991-1) used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human ORC2 Polyclonal Antibody | anti-ORC2 antibody
Rabbit anti-ORC2 Antibody, Affinity Purified
Gene Names
ORC2; ORC2L
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
ORC2, Antibody; Rabbit anti-ORC2 Antibody, Affinity Purified; ORC2L; origin recognition complex protein 2 homolog; origin recognition complex subunit 2; origin recognition complex, subunit 2 homolog; Origin recognition complex subunit 2; anti-ORC2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-ORC2 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 150 and 200
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human ORC2 by western blot. Samples: Whole cell lysate from HeLa (5, 15, and 50 ug) and HEK293T (T; 50 ug) cells. Antibody: Affinity purified rabbit anti-ORC2 antibody AAA211991 (lot AAA211991-1) used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human ORC2 by western blot. Samples: Whole cell lysate from HeLa (5, 15, and 50 ug) and HEK293T (T; 50 ug) cells. Antibody: Affinity purified rabbit anti-ORC2 antibody AAA211991 (lot AAA211991-1) used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human ORC2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ORC2 antibody AAA211991 (lot AAA211991-1) used for IP at 3 ug/mg lysate. ORC2 was also immunoprecipitated by rabbit anti-ORC2 antibody which recognizes an upstream epitope. For blotting immunoprecipitated ORC2, AAA211991 was used at 0.4 ug/ml. Detection: Chemiluminescence with exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human ORC2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ORC2 antibody AAA211991 (lot AAA211991-1) used for IP at 3 ug/mg lysate. ORC2 was also immunoprecipitated by rabbit anti-ORC2 antibody which recognizes an upstream epitope. For blotting immunoprecipitated ORC2, AAA211991 was used at 0.4 ug/ml. Detection: Chemiluminescence with exposure time of 30 seconds.)
IHC (Immunohistochemistry)
(Detection of human ORC2 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-ORC2 (Cat. No. AAA211991) used at a dilution of 1:200 (1ug/ml) Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human ORC2 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-ORC2 (Cat. No. AAA211991) used at a dilution of 1:200 (1ug/ml) Detection: DAB)
Related Product Information for anti-ORC2 antibody
The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. ORC2 is also known as ORC2L and is a subunit of the human ORC complex. ORC2 forms a core complex with ORC3L,-4L, and-5L [taken from NCBI Entrez Gene (GeneID: 4999)].
Product Categories/Family for anti-ORC2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
65,972 Da
NCBI Official Full Name
origin recognition complex subunit 2
NCBI Official Synonym Full Names
origin recognition complex, subunit 2
NCBI Official Symbol
ORC2
NCBI Official Synonym Symbols
ORC2L
NCBI Protein Information
origin recognition complex subunit 2; origin recognition complex protein 2 homolog; origin recognition complex, subunit 2 homolog
UniProt Protein Name
Origin recognition complex subunit 2
UniProt Gene Name
ORC2
UniProt Synonym Gene Names
ORC2L
UniProt Entry Name
ORC2_HUMAN
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Product Notes
The ORC2 orc2 (Catalog #AAA211991) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-ORC2 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ORC2 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the ORC2 orc2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ORC2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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