IHC (Immunohiostchemistry)
(OTOP1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of OTOP1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Rabbit OTOP1 Polyclonal Antibody | anti-OTOP1 antibody
OTOP1 Antibody (Center)
Reactivity
Mouse (Predicted Reactivity: Rat)
Applications
Immunohistochemistry, ELISA, Western Blot
Synonyms
OTOP1, Antibody; OTOP1 Antibody (Center); Otopetrin-1; OTOP1; anti-OTOP1 antibody
Host
Rabbit
Reactivity
Mouse (Predicted Reactivity: Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This OTOP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 360-388 amino acids from the Central region of human OTOP1.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
360-388
Sequence Length
612
Applicable Applications for anti-OTOP1 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Cellular Location
Cell membrane {ECO:0000250|UniProtKB:Q80VM9};Multi-pass membrane protein. Note=Detectedin the gelatinous membrane overlying theinner ear macular epithelium.{ECO:0000250|UniProtKB:Q80VM9
Function
Proton-selective channel that specificallytransports protons into cells (PubMed: ). Protonchannel activity is only weakly-sensitive tovoltage (By similarity). Proton-selectivechannel activity is probably required in celltypes that use changes in intracellular pH forcell signaling or to regulate biochemical ordevelopmental processes (PubMed: ). In thevestibular system of the inner ear, requiredfor the formation and function of otoconia,which are calcium carbonate crystals thatsense gravity and acceleration (By similarity).Probably acts by maintaining the pHappropriate for formation of otoconia (Bysimilarity). Regulates purinergic control ofintracellular calcium in vestibular supportingcells (By similarity). May be involved in sourtaste perception in sour taste cells bymediating entry of protons within the cytosol(By similarity). Also involved in energymetabolism, by reducing adipose tissueinflammation and protecting fromobesity-induced metabolic dysfunction (Bysimilarity).
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 Weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohiostchemistry)
(OTOP1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of OTOP1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(OTOP1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of OTOP1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(OTOP1 Antibody (Center) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the OTOP1 antibody detected the OTOP1 protein (arrow).)
WB (Western Blot)
(OTOP1 Antibody (Center) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the OTOP1 antibody detected the OTOP1 protein (arrow).)
Related Product Information for anti-OTOP1 antibody
OTOP1 is required for normal formation of otoconia in the inner ear. Inhibits P2Y purinoceptors. Modulates calcium homeostasis and influx of calcium in response to extracellular ATP (By similarity).
Product Categories/Family for anti-OTOP1 antibody
References
Hurle, B., et al. Hum. Mol. Genet. 12(7):777-789(2003)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
67353
NCBI Official Full Name
otopetrin-1
NCBI Official Synonym Full Names
otopetrin 1
NCBI Official Symbol
OTOP1
NCBI Protein Information
otopetrin-1
UniProt Protein Name
Otopetrin-1
UniProt Gene Name
OTOP1
UniProt Entry Name
OTOP1_HUMAN
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Product Notes
The OTOP1 otop1 (Catalog #AAA285832) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 360-388. The OTOP1 Antibody (Center) reacts with Mouse (Predicted Reactivity: Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's OTOP1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the OTOP1 otop1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "OTOP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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