Rabbit p130 Cas Polyclonal Antibody | anti-p130Cas antibody

p130 Cas Antibody

Cat. Num. AAA329551

• Gene Names: BCAR1; CAS; CAS1; CASS1; CRKAS; P130Cas
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (92%), Bovine (91%), Horse (92%), Sheep (92%), Rabbit (92%)
• Applications: ELISA, Western Blot
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
• Synonyms: p130 Cas, Antibody; p130 Cas Antibody; BCAR 1; Bcar1; BCAR1_HUMAN; Breast cancer anti estrogen resistance 1; Breast cancer anti estrogen resistance 1 protein; Breast cancer anti-estrogen resistance protein 1; CAS; Cas scaffolding protein family member 1; CAS1; Cass1; Crk associated substrate; Crk associated substrate p130Cas; CRK-associated substrate; CRKAS; FLJ12176; FLJ45059; p130cas; anti-p130Cas antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (92%), Bovine (91%), Horse (92%), Sheep (92%), Rabbit (92%)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: p130 Cas Antibody detects endogenous levels of total p130 Cas.; Tissue Specificity: Widely expressed with an abundant expression in the testis. Low level of expression seen in the liver, thymus, and peripheral blood leukocytes. The protein has been detected in a B-cell line.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-p130Cas antibody: ELISA (Peptide ELISA), WB (Western Blot)
• Immunogen: A synthesized peptide derived from human p130 Cas, corresponding to a region within N-terminal amino acids.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: PTK2/FAK1 activation mediates phosphorylation at the YDYVHL motif; phosphorylation is most likely catalyzed by SRC family members. SRC-family kinases are recruited to the phosphorylated sites and can phosphorylate other tyrosine residues. Tyrosine phosphorylation is triggered by integrin-mediated adhesion of cells to the extracellular matrix.Dephosphorylated by PTPN14 at Tyr-128.
• Subunit Structure: Forms complexes in vivo with PTK2/FAK1, adapter protein CRKL and LYN kinase. Can heterodimerize with NEDD9. Interacts with BCAR3, the interaction regulates adhesion-dependent serine phosphorylation. Interacts with NPHP1 and SH2D3C. Interacts with activated CSPG4. Interacts with BMX, INPPL1/SHIP2 and PEAK1. Part of a collagen-stimulated complex involved in cell migration made of CDC42, CRK, TNK2 and BCAR1/p130cas. Interacts with TNK2 via SH3 domains. Interacts with PTK2B/PYK2.
• Similarity: Contains a central domain (substrate domain) containing multiple potential SH2-binding sites and a C-terminal domain containing a divergent helix-loop-helix (HLH) motif. The SH2-binding sites putatively bind CRK, NCK and ABL1 SH2 domains. The HLH motif is absolutely required for the induction of pseudohyphal growth in yeast and mediates heterodimerization with NEDD9 (By similarity).A serine-rich region promotes activation of the serum response element (SRE).The SH3 domain is necessary for the localization of the protein to focal adhesions and interacts with one proline-rich region of PTK2/FAK11.Belongs to the CAS family.
• Subcellular Location: Cell junction>Focal adhesion. Cytoplasm.; Note: Unphosphorylated form localizes in the cytoplasm and can move to the membrane upon tyrosine phosphorylation.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

WB (Western Blot)

(Western blot analysis of p130 Cas in lysates of NIH-3T3, using p130 Cas Antibody(AF4748).)

WB (Western Blot)

(Western blot analysis of extracts from A2780 cells, using p130 Cas Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-p130Cas antibody

Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. Overexpression confers antiestrogen resistance on breast cancer cells.

NCBI and Uniprot Product Information

• NCBI GI #: 282398112
• NCBI GeneID: 9564
• NCBI Accession #: NP_001164185.1
• NCBI GenBank Nucleotide #: NM_001170714.1
• UniProt Accession #: P56945; Q6QEF7; B4DIW5; B7Z7X7; F5GXV6; F8WA69
• Molecular Weight: 93,372 Da
• NCBI Official Full Name: breast cancer anti-estrogen resistance protein 1 isoform 1
• NCBI Official Synonym Full Names: breast cancer anti-estrogen resistance 1
• NCBI Official Symbol: BCAR1
• NCBI Official Synonym Symbols: CAS; CAS1; CASS1; CRKAS; P130Cas
• NCBI Protein Information: breast cancer anti-estrogen resistance protein 1; Crk-associated substrate p130Cas; Cas scaffolding protein family member 1
• UniProt Protein Name: Breast cancer anti-estrogen resistance protein 1
• UniProt Gene Name: BCAR1
• UniProt Synonym Gene Names: CAS; CASS1; CRKAS
• UniProt Entry Name: BCAR1_HUMAN