Rabbit p21 Cip1 Polyclonal Antibody | anti-p21Cip1 antibody

Phospho-p21 Cip1 (Thr57) Antibody

Cat. Num. AAA329948

• Gene Names: CDKN1A; P21; CIP1; SDI1; WAF1; CAP20; CDKN1; MDA-6; p21CIP1
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (80%), Xenopus (80%)
• Applications: ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
• Purity: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Synonyms: p21 Cip1, Antibody; Phospho-p21 Cip1 (Thr57) Antibody; CAP20; CDK-interacting protein 1; CDKI; CDKN1; Cdkn1a; CDN1A_HUMAN; CIP1; Cyclin Dependent Kinase Inhibitor 1A; Cyclin-dependent kinase inhibitor 1; Cyclin-dependent kinase inhibitor 1A (P21); Cyclin-dependent kinase inhibitor 1A (p21, Cip1); DNA Synthesis Inhibitor; MDA-6; MDA6; Melanoma differentiation-associated protein 6; Melanoma differentiation-associated protein; p21; P21 protein; p21CIP1; p21Cip1/Waf1; p21WAF; PIC1; SDI1; SLC12A9; WAF1; Wild type p53 activated fragment 1 (WAF1); Wild type p53 activated fragment 1; Wildtype p53-activated fragment 1; anti-p21Cip1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (80%), Xenopus (80%)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Phospho-p21 Cip1 (Thr57) Antibody detects endogenous levels of p21 Cip1 only when phosphorylated at Thr57.; Tissue Specificity: Expressed in all adult tissues, with 5-fold lower levels observed in the brain.
• Purity/Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-p21Cip1 antibody: ELISA (Peptide ELISA), ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
• Immunogen: A synthesized peptide derived from human p21 Cip1 around the phosphorylation site of Thr57.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: Phosphorylation of Thr-145 by Akt or of Ser-146 by PKC impairs binding to PCNA. Phosphorylation at Ser-114 by GSK3-beta enhances ubiquitination by the DCX (DTL) complex. Phosphorylation of Thr-145 by PIM2 enhances CDKN1A stability and inhibits cell proliferation. Phosphorylation of Thr-145 by PIM1 results in the relocation of CDKN1A to the cytoplasm and enhanced CDKN1A protein stability. UV radiation-induced phosphorylation at Thr-80 by LKB1 and at Ser-146 by NUAK1 leads to its degradation.Ubiquitinated by MKRN1; leading to polyubiquitination and 26S proteasome-dependent degradation. Ubiquitinated by the DCX (DTL) complex, also named CRL4 (CDT2) complex, leading to its degradation during S phase or following UV irradiation. Ubiquitination by the DCX (DTL) complex is essential to control replication licensing and is PCNA-dependent: interacts with PCNA via its PIP-box, while the presence of the containing the 'K+4' motif in the PIP box, recruit the DCX (DTL) complex, leading to its degradation. Ubiquitination at Ser-2 leads to degradation by the proteasome pathway. Ubiquitinated by RNF114; leading to proteasomal degradation. Acetylation leads to protein stability. Acetylated in vitro on Lys-141, Lys-154, Lys-161 and Lys-163. Deacetylation by HDAC1 is prevented by competitive binding of C10orf90/FATS to HDAC1 (By similarity).
• Subunit Structure: Interacts with HDAC1; the interaction is prevented by competitive binding of C10orf90/FATS to HDAC1 facilitating acetylation and protein stabilization of CDKN1A/p21 (By similarity). Interacts with MKRN1. Interacts with PSMA3. Interacts with PCNA. Component of the ternary complex, cyclin D-CDK4-CDKN1A. Interacts (via its N-terminal domain) with CDK4; the interaction promotes the assembly of the cyclin D-CDK4 complex, its nuclear translocation and promotes the cyclin D-dependent enzyme activity of CDK4. Binding to CDK2 leads to CDK2/cyclin E inactivation at the G1-S phase DNA damage checkpoint, thereby arresting cells at the G1-S transition during DNA repair. Interacts with PIM1. Interacts with STK11 and NUAK1. Interacts wih DTL. Interacts with isoform 1 and isoform 2 of TRIM39.
• Similarity: The PIP-box K+4 motif mediates both the interaction with PCNA and the recruitment of the DCX (DTL) complex: while the PIP-box interacts with PCNA, the presence of the K+4 submotif, recruits the DCX (DTL) complex, leading to its ubiquitination.The C-terminal is required for nuclear localization of the cyclin D-CDK4 complex.Belongs to the CDI family.
• Subcellular Location: Cytoplasm. Nucleus.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

IF (Immunofluorescence)

(Staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot)

(Western blot analysis of p21Cip1 (Phospho-Thr57) using TNF-α treated HeLa whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)

Related Product Information for anti-p21Cip1 antibody

May be involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. Inhibits DNA synthesis by DNA polymerase delta by competing with POLD3 for PCNA binding. Plays an important role in controlling cell cycle progression and DNA damage-induced G2 arrest.

NCBI and Uniprot Product Information

• NCBI GI #: 11386203
• NCBI GeneID: 1026
• NCBI Accession #: NP_000380.1
• NCBI GenBank Nucleotide #: NM_000389.4
• UniProt Accession #: P38936; Q14010; Q9BUT4
• Molecular Weight: 18,119 Da
• NCBI Official Full Name: cyclin-dependent kinase inhibitor 1
• NCBI Official Synonym Full Names: cyclin-dependent kinase inhibitor 1A (p21, Cip1)
• NCBI Official Symbol: CDKN1A
• NCBI Official Synonym Symbols: P21; CIP1; SDI1; WAF1; CAP20; CDKN1; MDA-6; p21CIP1
• NCBI Protein Information: cyclin-dependent kinase inhibitor 1; DNA synthesis inhibitor; CDK-interacting protein 1; CDK-interaction protein 1; wild-type p53-activated fragment 1; melanoma differentiation associated protein 6
• UniProt Protein Name: Cyclin-dependent kinase inhibitor 1
• UniProt Gene Name: CDKN1A
• UniProt Synonym Gene Names: CAP20; CDKN1; CIP1; MDA6; PIC1; SDI1; WAF1; MDA-6
• UniProt Entry Name: CDN1A_HUMAN