Rabbit anti-Human p38 Polyclonal Antibody | anti-MAPK14 antibody

Anti-p38 Antibody, Rabbit Polyclonal

Cat. Num. AAA254357

• Gene Names: MAPK14; RK; p38; CSBP; EXIP; Mxi2; CSBP1; CSBP2; CSPB1; PRKM14; PRKM15; SAPK2A; p38ALPHA
• Reactivity: Human
• Applications: Immunoprecipitation, Immunohistochemistry, ELISA, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: p38, Antibody; Anti-p38 Antibody, Rabbit Polyclonal; p38 alpha/MAPK14 Antibody, Rabbit PAb, Antigen Affinity Purified; mitogen-activated protein kinase 14; Anti-CSBP Antibody; Anti-CSBP1 Antibody; Anti-CSBP2 Antibody; Anti-CSPB1 Antibody; Anti-EXIP Antibody; Anti-Mxi2 Antibody; Anti-p38 Antibody; Anti-p38ALPHA Antibody; Anti-PRKM14 Antibody; Anti-PRKM15 Antibody; Anti-RK Antibody; Anti-SAPK2A Antibody; anti-MAPK14 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human p38 alpha/MAPK14
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-MAPK14 antibody: IP (Immunoprecipitation), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
• Immunogen: Recombinant Human p38 alpha/MAPK14 protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human p38 alpha/MAPK14 (rh p38 alpha/MAPK14). p38 alpha/MAPK14 specific IgG was purified by Human p38 alpha/MAPK14 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

WB (Western Blot)

(Anti-MAPK14 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLane B: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:41 kDaObserved band size:39 kDa)

IP (Immunoprecipitation)

(MAPK14 was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell LysateLane B:0.5 mg Jurkat Whole Cell LysateLane C:0.5 mg 293T Whole Cell LysateLane D:0.5 mg MCF-7 Whole Cell Lysate2 uL anti-MAPK14 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-MAPK14 rabbit polyclonal antibody,at 1:330 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 38 kDaObserved band size: 38 kDa)

IHC (Immunohistochemisry)

(Immunochemical staining of human MAPK14 in human brain with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

IHC (Immunohiostchemistry)

(Immunochemical staining of human MAPK14 in human placenta with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

IHC (Immunohistochemistry)

(Immunochemical staining of human MAPK14 in human skeletal muscle with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

Related Product Information for anti-MAPK14 antibody

MAPK14 contains 1 protein kinase domain and belongs to the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation, and development. MAPK14 can be detected in the brain, heart, placenta, pancreas, and skeletal muscle and it is expressed to a lesser extent in the lung, liver, and kidney. MAPK14 is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with MAPK14. The substrates of p38 alpha include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of p38 alpha in stress-related transcription and cell cycle regulation, as well as in genotoxic stress response. In response to activation by environmental stress, pro-inflammatory cytokines, and lipopolysaccharide, MAPK14 phosphorylates some transcription factors, such as ELK1 and ATF2, and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. MAPK14 plays a critical role in the production of some cytokines, for example, IL-6. It may play a role in the stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2.

Product Categories/Family for anti-MAPK14 antibody

Non-Canonical Wnt Pathway

References

Luo X, et al. (2011) Study on p38 mitogen activated protein kinase in vascular endothelial cells dysfunction in preeclampsia. Zhonghua Fu Chan Ke Za Zhi. 46(1):36-40.Park CH, et al. (2011) Epidermal growth factor-induced matrix metalloproteinase-1 expression is negatively regulated by p38 MAPK in human skin fibroblasts. J Dermatol Sci. 64(2):134-41.Lee JY, et al. (2011) Curcumin induces EGFR degradation in lung adenocarcinoma and modulates p38 activation in intestine: the versatile adjuvant for gefitinib therapy. PLoS One. 6(8):e23756.Riis JL, et al. (2011) CCL27 expression is regulated by both p38 MAPK and IKKbeta signalling pathways. Cytokine. 56(3):699-707.

NCBI and Uniprot Product Information

• NCBI GI #: 194578900
• NCBI GeneID: 1432
• NCBI Accession #: NP_620581.1
• NCBI GenBank Nucleotide #: NM_139012
• UniProt Accession #: Q16539; O60776; Q13083; Q14084; Q8TDX0; A6ZJ92; A8K6P4; B0LPH0
• Molecular Weight: ~65 kDa
• NCBI Official Full Name: Homo sapiens mitogen-activated protein kinase 14 (MAPK14), transcript variant 2, mRNA
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 14
• NCBI Official Symbol: MAPK14
• NCBI Official Synonym Symbols: RK; p38; CSBP; EXIP; Mxi2; CSBP1; CSBP2; CSPB1; PRKM14; PRKM15; SAPK2A; p38ALPHA
• NCBI Protein Information: mitogen-activated protein kinase 14; MAP kinase 14; p38alpha Exip; p38 MAP kinase; MAP kinase Mxi2; MAP kinase p38 alpha; CSAID-binding protein; Csaids binding protein; MAX-interacting protein 2; stress-activated protein kinase 2A; p38 mitogen activated p
• UniProt Protein Name: Mitogen-activated protein kinase 14
• UniProt Gene Name: MAPK14
• UniProt Synonym Gene Names: CSBP; CSBP1; CSBP2; CSPB1; MXI2; SAPK2A; MAP kinase 14; MAPK 14; CSAID-binding protein; CSBP; MAP kinase p38 alpha; SAPK2a
• UniProt Entry Name: MK14_HUMAN