Rabbit anti-Human P38 Delta/MAPK13 Polyclonal Antibody | anti-MAPK13 antibody

Anti-P38 Delta/MAPK13 Antibody, Rabbit Polyclonal

Cat. Num. AAA255136

• Gene Names: MAPK13; SAPK4; PRKM13; MAPK 13; MAPK-13; p38delta
• Reactivity: Human
• Applications: Immunoprecipitation, ELISA, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: P38 Delta/MAPK13, Antibody; Anti-P38 Delta/MAPK13 Antibody, Rabbit Polyclonal; p38 delta/MAPK13 Antibody, Rabbit PAb, Antigen Affinity Purified; mitogen-activated protein kinase 13; Anti-MAPK-13 Antibody; Anti-MAPK13 Antibody; Anti-p38delta Antibody; Anti-PRKM13 Antibody; Anti-SAPK4 Antibody; anti-MAPK13 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human p38 delta/MAPK13
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-MAPK13 antibody: IP (Immunoprecipitation), ELISA, WB (Western Blot)
• Immunogen: Recombinant Human p38 delta/MAPK13 Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human p38 delta/MAPK13 (rh p38 delta/MAPK13). p38 delta/MAPK13 specific IgG was purified by Human p38 delta/MAPK13 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

WB (Western Blot)

(Anti-MAPK13 rabbit polyclonal antibody at 1:500 dilutionLane A: 293 Whole Cell LysateLane B: MCF7 Whole Cell LysateLane C: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:42 kDaObserved band size:44 kDa(We are unsure as to the identity of these extra bands.))

IP (Immunoprecipitation)

(MAPK13 was immunoprecipitated using:Lane A:0.5 mg MCF-7 Whole Cell LysateLane B:0.5 mg HepG2 Whole Cell Lysate4 uL anti-MAPK13 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-MAPK13 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 43 kDaObserved band size: 43 kDa)

Related Product Information for anti-MAPK13 antibody

The p38 family of mitogen-activated protein kinases (MAPK) includes p38 alpha (SAPK2a, CSBP), p38 beta (SAPK2b), p38 delta (SAPK4), and p38 gamma (SAPK3/ERK6). p38 alpha and p38 beta are widely expressed p38 isoforms that are involved in the regulation of cell proliferation, differentiation, development, and stress response. p38 delta, also known as MAPK13, is a regulator of differentiation-dependent gene expression in keratinocytes and has been a regulator of surface epithelial differentiation and apoptosis. p38 delta protein is upregulated in Cholangiocarcinoma (CC) relative to hepatocellular carcinoma (HCC) and normal biliary tract tissues. p38 delta is important for motility and invasion of CC cells, suggesting that p38 delta may play an important role in CC metastasis. p38 delta is expressed in the epidermis, suggesting a role for p38 delta in regulating differentiation. p38 delta is the major p38 isoform driving suprabasal involucrin gene expression and that p38 delta directly regulates ERK1/2 activity via the formation of a p38 delta-ERK1/2 complex. Recent emerging evidence suggests that the p38 stress MAPK pathway may function as a tumor suppressor through regulating Ras-dependent and -independent proliferation, transformation, invasion, and cell death by isoform-specific mechanisms. p38 delta has an important role in promoting cell proliferation and tumor development in the epidermis and may have therapeutic implications for skin cancer.

Product Categories/Family for anti-MAPK13 antibody

VEGF Signaling Pathway

References

Efimova T, et al. (2003) A regulatory role for p38 delta MAPK in keratinocyte differentiation. Evidence for p38 delta-ERK1/2 complex formation. J Biol Chem. 278(36): 34277-85.Eckert RL, et al. (2003) p38 Mitogen-activated protein kinases on the body surface--a function for p38 delta. J Invest Dermatol. 120(5): 823-8.Loesch M, et al. (2008) The p38 MAPK stress pathway as a tumor suppressor or more? Front Biosci. 13: 3581-93.Schindler EM, et al. (2009) p38delta Mitogen-activated protein kinase is essential for skin tumor development in mice. Cancer Res. 69(11): 4648-55.Tan FL, et al. (2010) p38delta/MAPK13 as a diagnostic marker for cholangiocarcinoma and its involvement in cell motility and invasion. Int J Cancer. 126(10): 2353-61.

NCBI and Uniprot Product Information

• NCBI GI #: 4506085
• NCBI GeneID: 5603
• NCBI Accession #: NP_002745.1
• NCBI GenBank Nucleotide #: NM_002754.4
• UniProt Accession #: O15264; O14739; O15124; Q5U4A5; Q6FI46; Q9UNU0
• Molecular Weight: 28,779 Da
• NCBI Official Full Name: mitogen-activated protein kinase 13
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 13
• NCBI Official Symbol: MAPK13
• NCBI Official Synonym Symbols: SAPK4; PRKM13; MAPK 13; MAPK-13; p38delta
• NCBI Protein Information: mitogen-activated protein kinase 13; MAP kinase 13; MAP kinase p38 delta; mitogen-activated protein kinase p38 delta; stress-activated protein kinase 4
• UniProt Protein Name: Mitogen-activated protein kinase 13
• UniProt Gene Name: MAPK13
• UniProt Synonym Gene Names: PRKM13; SAPK4; MAP kinase 13; MAPK 13; MAP kinase p38 delta
• UniProt Entry Name: MK13_HUMAN