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WB (Western Blot)
(Detection of human P53 by western blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-P53 antibody AAA210839 (lot AAA210839-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human p53 Polyclonal Antibody | anti-p53 antibody
Rabbit anti-p53 Antibody, Affinity Purified
Gene Names
TP53; P53; BCC7; LFS1; TRP53
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Chromatin Immunoprecipitation, Immunoprecipitation, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
p53, Antibody; Rabbit anti-p53 Antibody, Affinity Purified; phosphoprotein p53; tumor supressor p53; Tumor suppressor p53; tumor protein 53; TRP53; transformation-related protein 53; P53; mutant tumor protein 53; LFS1; cellular tumor antigen p53; BCC7; antigen NY-CO-13; p53 tumor suppressor; Cellular tumor antigen p53; tumor protein p53; anti-p53 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-p53 antibody
FCM/FACS (Flow Cytometry), ChIP (Chromatin immunoprecipitation), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 50 and 100
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human P53 by western blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-P53 antibody AAA210839 (lot AAA210839-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human P53 by western blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-P53 antibody AAA210839 (lot AAA210839-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human P53 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-P53 antibody AAA210839 (lot AAA210839-2) used for IP at 3 ug per reaction. P53 was also immunoprecipitated by rabbit anti-P53 antibody For blotting immunoprecipitated P53, AAA210839 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human P53 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-P53 antibody AAA210839 (lot AAA210839-2) used for IP at 3 ug per reaction. P53 was also immunoprecipitated by rabbit anti-P53 antibody For blotting immunoprecipitated P53, AAA210839 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IHC (Immunohistochemisry)
(Detection of human p53 by immunohistochemistry. Sample: FFPE section of human lung cancer Antibody: Affinity purified rabbit anti-p53 (Cat. No. AAA210839 Lot2) used at 1:5,000 (0.2ug/ml). Detection: DAB)
IHC (Immunohistochemisry)
(Detection of human p53 by immunohistochemistry. Sample: FFPE section of human lung cancer Antibody: Affinity purified rabbit anti-p53 (Cat. No. AAA210839 Lot2) used at 1:5,000 (0.2ug/ml). Detection: DAB)
FCM/FACS (Flow Cytometry)
(Flow Cytometrical Analysis of p53 in Jurkat Cells. Jurkat cells were fixed in 1.5% PFA, and permeabilized in 90% Methanol. 1 X 10^6 cells were stained with 1.5 ug anti-KLH IgG control or anti-p53 [AAA210839] and secondary FITC-conjugated goat anti-rabbit (in a 150ul reaction). anti-KLH control IgG (black); anti-p53 (red).)
FCM/FACS (Flow Cytometry)
(Flow Cytometrical Analysis of p53 in Jurkat Cells. Jurkat cells were fixed in 1.5% PFA, and permeabilized in 90% Methanol. 1 X 10^6 cells were stained with 1.5 ug anti-KLH IgG control or anti-p53 [AAA210839] and secondary FITC-conjugated goat anti-rabbit (in a 150ul reaction). anti-KLH control IgG (black); anti-p53 (red).)
ChIP (Chromatin Immunoprecipitation)
(Localization of p53 Binding Sites by ChIP-sequencing. Chromatin from MCF-7 was immunoprecipitated with anti-p53 antibody AAA210839 and analyzed by DNA sequencing. The figure illustrates the peak distribution of p53 binding within a 250 Kb region of chromosome 6 as detected using anti-p53 AAA210839. ChIP-seq validation performed by Active Motif, Carlsbad, CA.)
ChIP (Chromatin Immunoprecipitation)
(Localization of p53 Binding Sites by ChIP-sequencing. Chromatin from MCF-7 was immunoprecipitated with anti-p53 antibody AAA210839 and analyzed by DNA sequencing. The figure illustrates the peak distribution of p53 binding within a 250 Kb region of chromosome 6 as detected using anti-p53 AAA210839. ChIP-seq validation performed by Active Motif, Carlsbad, CA.)
Related Product Information for anti-p53 antibody
p53 is a tumor suppressor protein that is mutated or inactivated in over 50% of human cancers. Loss of function defects in p53 are the cause of the autosomal dominant familial cancer syndrome, Li-Fraumeni syndrome (LFS) that is characterized by the development of a diverse set of malignancies at very early ages. At the cellular level p53 is involved in the negative regulation of cell the cycle via its transactivational control of genes required for cell cycle progression. Depending on the physiological circumstance, p53 can promote growth arrest or apoptosis.
Product Categories/Family for anti-p53 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
43,653 Da
NCBI Official Full Name
cellular tumor antigen p53 isoform a
NCBI Official Synonym Full Names
tumor protein p53
NCBI Official Symbol
TP53
NCBI Official Synonym Symbols
P53; BCC7; LFS1; TRP53
NCBI Protein Information
cellular tumor antigen p53; antigen NY-CO-13; phosphoprotein p53; p53 tumor suppressor; transformation-related protein 53
UniProt Protein Name
Cellular tumor antigen p53
UniProt Gene Name
TP53
UniProt Synonym Gene Names
P53
UniProt Entry Name
P53_HUMAN
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Product Notes
The p53 tp53 (Catalog #AAA210839) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-p53 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's p53 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ChIP (Chromatin immunoprecipitation), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the p53 tp53 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p53, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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