Rabbit anti-Human p53 Polyclonal Antibody | anti-p53 antibody

p53 (Phospho-Ser315) Antibody

Cat. Num. AAA302943

• Gene Names: TP53; P53; BCC7; LFS1; TRP53
• Reactivity: Human
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Synonyms: p53, Antibody; p53 (Phospho-Ser315) Antibody; Tumor suppressor p53; Phosphoprotein p53; Antigen NY-CO-13; TP53; anti-p53 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 393

• Applicable Applications for anti-p53 antibody: IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen Type: Peptide-KLH
• Immunogen Description: Peptide sequence around phosphorylation site of serine 315 (S-S-S(p)-P-Q) derived from Human p53.
• Modification: Phospho-Ser315
• Target Name: p53
• Preparation and Storage: Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.

IF (Immunofluorescence)

(Immunofluorescence staining of methanol-fixed Hela cells using p53(Phospho-Ser315) Antibody.)

IHC (Immunohiostchemistry)

(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p53(Phospho-Ser315) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot)

(Western blot analysis of extracts from Hela cells using p53(Phospho-Ser315) Antibody (Lane 2) and the same antibody preincubated with blocking peptide(Lane1).)

Related Product Information for anti-p53 antibody

Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Implicated in Notch signaling cross-over.

Product Categories/Family for anti-p53 antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 120407068
• NCBI GeneID: 7157
• NCBI Accession #: NP_000537.3
• NCBI GenBank Nucleotide #: NM_000546.5
• UniProt Accession #: P04637; Q15086; Q15087; Q15088; Q16535; Q16807; Q16808; Q16809; Q16810; Q16811; Q16848; Q2XN98
• Molecular Weight: 24,401 Da
• NCBI Official Full Name: cellular tumor antigen p53 isoform a
• NCBI Official Synonym Full Names: tumor protein p53
• NCBI Official Symbol: TP53
• NCBI Official Synonym Symbols: P53; BCC7; LFS1; TRP53
• NCBI Protein Information: cellular tumor antigen p53
• UniProt Protein Name: Cellular tumor antigen p53
• UniProt Gene Name: TP53
• UniProt Synonym Gene Names: P53
• UniProt Entry Name: P53_HUMAN