IHC (Immunohistochemisry)
(p53 Antibody (T55) immunohistochemistry analysis in formalin fixed and paraffin embedded human skin tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of p53 Antibody (T55) for immunohistochemistry. Clinical relevance has not been evaluated.)
Rabbit anti-Human p53 Polyclonal Antibody | anti-TP53 antibody
p53 Antibody (T55)
Gene Names
TP53; P53; BCC7; LFS1; TRP53
Reactivity
Human
Applications
Immunohistochemistry, Immunofluorescence, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
p53, Antibody; p53 Antibody (T55); Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53; TP53; P53; anti-TP53 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This p53 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 33-62 amino acids from human p53.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.3 (varies by lot)
Sequence Positions
33-62
Sequence Length
393
Applicable Applications for anti-TP53 antibody
IHC (Immunohistochemistry), IF (Immunofluorescence), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistochemisry)
(p53 Antibody (T55) immunohistochemistry analysis in formalin fixed and paraffin embedded human skin tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of p53 Antibody (T55) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohistochemisry)
(p53 Antibody (T55) immunohistochemistry analysis in formalin fixed and paraffin embedded human skin tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of p53 Antibody (T55) for immunohistochemistry. Clinical relevance has not been evaluated.)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of p53 Antibody (T55) with A2058 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of p53 Antibody (T55) with A2058 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)
WB (Western Blot)
(Western blot analysis of TP53(arrow) using rabbit polyclonal p53 Antibdoy (T55). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the TP53 gene (Lane 2) (Origene Technologies).)
WB (Western Blot)
(Western blot analysis of TP53(arrow) using rabbit polyclonal p53 Antibdoy (T55). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the TP53 gene (Lane 2) (Origene Technologies).)
Related Product Information for anti-TP53 antibody
Tumor protein p53, a nuclear protein, plays an essential role in the regulation of cell cycle, specifically in the transition from G0 to G1. It is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing DNA-binding, oligomerization and transcription activation domains. It is postulated to bind as a tetramer to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of the TP53 gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome.
Product Categories/Family for anti-TP53 antibody
References
Blanchette, P., et al., Mol. Cell. Biol. 24(21):9619-9629 (2004).
Adachi, K., et al., Oncogene 23(47):7791-7798 (2004).
Zhang, Y., et al., J. Biol. Chem. 279(41):42545-42551 (2004).
Anazawa, Y., et al., Oncogene 23(46):7621-7627 (2004).
Montagnoli, A., et al., Cancer Res. 64(19):7110-7116 (2004).
Adachi, K., et al., Oncogene 23(47):7791-7798 (2004).
Zhang, Y., et al., J. Biol. Chem. 279(41):42545-42551 (2004).
Anazawa, Y., et al., Oncogene 23(46):7621-7627 (2004).
Montagnoli, A., et al., Cancer Res. 64(19):7110-7116 (2004).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
43653
NCBI Official Full Name
cellular tumor antigen p53 isoform a
NCBI Official Synonym Full Names
tumor protein p53
NCBI Official Symbol
TP53
NCBI Official Synonym Symbols
P53; BCC7; LFS1; TRP53
NCBI Protein Information
cellular tumor antigen p53
UniProt Protein Name
Cellular tumor antigen p53
UniProt Gene Name
TP53
UniProt Synonym Gene Names
P53
UniProt Entry Name
P53_HUMAN
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Product Notes
The TP53 tp53 (Catalog #AAA286241) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 33-62. The p53 Antibody (T55) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's p53 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IF (Immunofluorescence), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the TP53 tp53 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p53, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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