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IP (Immunoprecipitation)
(PABP was immunoprecipitated using: Lane A:0.5 mg SW480 Whole Cell Lysate 4 uL anti- PABP rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti- PABP rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 71 kDa Observed band size :80 kDa)
Rabbit anti-Human PABPC1 Polyclonal Antibody | anti-PABPC1 antibody
Anti-PABPC1 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunoprecipitation, Immunohistochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
PABPC1, Antibody; Anti-PABPC1 Antibody, Rabbit Polyclonal; PABPC1 Antibody, Rabbit PAb, Antigen Affinity Purified; Anti-PAB1 Antibody; Anti-PABP Antibody; Anti-PABP1 Antibody; Anti-PABPC2 Antibody; Anti-PABPL1 Antibody; poly(A) binding protein, cytoplasmic 1; anti-PABPC1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human PABPC1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-PABPC1 antibody
IP (Immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot)
Conjugation
Unconjugated
Immunogen
A synthetic peptide corresponding to the center region of the Human PABPC1
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human PABPC1, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2 degree C-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(PABP was immunoprecipitated using: Lane A:0.5 mg SW480 Whole Cell Lysate 4 uL anti- PABP rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti- PABP rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 71 kDa Observed band size :80 kDa)
IP (Immunoprecipitation)
(PABP was immunoprecipitated using: Lane A:0.5 mg SW480 Whole Cell Lysate 4 uL anti- PABP rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti- PABP rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 71 kDa Observed band size :80 kDa)
IHC (Immunohistochemisry)
(Immunochemical staining of human PABPC1 in human colon carcinoma with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemisry)
(Immunochemical staining of human PABPC1 in human colon carcinoma with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohiostchemistry)
(Immunochemical staining of human PABPC1 in human lung cancer with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohiostchemistry)
(Immunochemical staining of human PABPC1 in human lung cancer with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
WB (Western Blot)
(Anti-PABPC1 rabbit polyclonal antibody at 1:500 dilution Lane A: H1299 Whole Cell Lysate Lane B: MCF7 Whole Cell Lysate Lane C: SW480 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:71 kDa)
WB (Western Blot)
(Anti-PABPC1 rabbit polyclonal antibody at 1:500 dilution Lane A: H1299 Whole Cell Lysate Lane B: MCF7 Whole Cell Lysate Lane C: SW480 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:71 kDa)
Related Product Information for anti-PABPC1 antibody
Polyadenylate-binding protein cytoplasmic 1 (PABPC1) is a cytoplasmic-nuclear shuttling protein important for protein translation initiation and both RNA processing and stability. PABPC1 controls the rate of mRNA deadenylation and participates in mRNA decay, which is involved in carcinogenesis. PABPC1 is an important cellular target of viral ORF57 to directly upregulate PAN accumulation during viral lytic infection, and the ability of host PABPC1 to disrupt ORF57 expression is a strategic host counterbalancing mechanism. Embryonic poly(A)-binding protein (EPAB) and poly(A)-binding protein, cytoplasmic 1 (PABPC1) bind poly(A) tails of mRNAs and mediate their translational regulation in germ cells and early preimplantation embryos. PABPC1 gene was identified as a downstream target of SNHG14, and PABPC1 mediates the SNHG14-induced oncogenic effects.
References
Ozturk S, et al. (2015) Epab and pabpc1 are differentially expressed in the postnatal mouse ovaries. J Assist Reprod Genet 32 (1): 137-146.Massimelli MJ, et al. (2013) Interplay between polyadenylate-binding protein 1 and kaposi's sarcoma-associated herpesvirus orf57 in accumulation of polyadenylated nuclear rna, a viral long noncoding rna. J Virol 87 (1): 243-256.Dong H, et al. (2018) Long non-coding rna snhg14 induces trastuzumab resistance of breast cancer via regulating pabpc1 expression through h3k27 acetylation. J Cell Mol Med 22 (10): 4935-4947.
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Product Notes
The PABPC1 (Catalog #AAA258516) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PABPC1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PABPC1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the PABPC1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PABPC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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