Rabbit anti-Human PABPN1 Polyclonal Antibody | anti-PABPN1 antibody

Rabbit anti-PABPN1 Antibody, Affinity Purified

Cat. Num. AAA212207

• Gene Names: PABPN1; OPMD; PAB2; PABII; PABP2; PABP-2
• Reactivity: Human
• Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Antigen Affinity Purified
• Synonyms: PABPN1, Antibody; Rabbit anti-PABPN1 Antibody, Affinity Purified; Nuclear poly(A)-binding protein 1; poly(A) binding protein II; Polyadenylate-binding nuclear protein 1; polyadenylate-binding protein 2; Poly(A)-binding protein II; Poly(A)-binding protein 2; PABP-2; PABP2; PABII; OPMD; poly(A) binding protein 2; PAB2; Polyadenylate-binding protein 2; poly(A) binding protein nuclear 1; anti-PABPN1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Purity/Purification: Antigen Affinity Purified
• Form/Format: Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
• Concentration: 200ug/ml (varies by lot)

• Applicable Applications for anti-PABPN1 antibody: IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
• Immunogen: Between 100 and 150
• Conjugation: Unconjugated
• Preparation and Storage: Store at 2-8 degree C; Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of human PABPN1 by western blot. Samples: Whole cell lysate from HeLa (15 and 50 ug), HEK293T (T; 50 ug), and Jurkat (J; 50 ug) cells. Antibody: Affinity purified rabbit anti-PABPN1 antibody AAA212207 (lot AAA212207-1) used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)

IP (Immunoprecipitation)

(Detection of human PABPN1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PABPN1 antibody AAA212207 (lot AAA212207-1) used for IP at 6 ug/mg lysate. PABPN1 was also immunoprecipitated by rabbit anti-PABPN1 antibody which recognizes an upstream epitope. For blotting immunoprecipitated PABPN1, AAA212207 was used at 0.4 ug/ml. Detection: Chemiluminescence with exposure time of 1 second.)

IHC (Immunohistochemistry)

(Detection of human PABPN1 by immunohistochemistry. Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-PABPN1 (Cat. No. AAA212207 Lot1) used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB. Counterstain: hematoxylin (blue).)

Related Product Information for anti-PABPN1 antibody

Polyadenylate binding protein, nuclear 1 (PABPN1) is involved in the 3'-end formation of mRNA precursors (pre-mRNA) by the addition of a poly(A) tail of 200-250 nt to the upstream cleavage product. PABPN1 stimulates poly(A) polymerase (PAPOLA) conferring processivity on the poly(A) tail elongation reaction and also controls the poly(A) tail length. PABPN1 increases the affinity of poly(A) polymerase for RNA and is also present at various stages of mRNA metabolism including nucleocytoplasmic trafficking and nonsense-mediated decay (NMD) of mRNA [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q86U42].

Product Categories/Family for anti-PABPN1 antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 4758876
• NCBI GeneID: 8106
• NCBI Accession #: NP_004634.1
• NCBI GenBank Nucleotide #: NM_004643.3
• UniProt Accession #: Q86U42; O43484; D3DS49
• Molecular Weight: 32749 MW
• NCBI Official Full Name: polyadenylate-binding protein 2
• NCBI Official Synonym Full Names: poly(A) binding protein nuclear 1
• NCBI Official Symbol: PABPN1
• NCBI Official Synonym Symbols: OPMD; PAB2; PABII; PABP2; PABP-2
• NCBI Protein Information: polyadenylate-binding protein 2
• UniProt Protein Name: Polyadenylate-binding protein 2
• UniProt Gene Name: PABPN1
• UniProt Synonym Gene Names: PAB2; PABP2; PABP-2; Poly(A)-binding protein 2; PABII