WB (Western Blot)
(Figure 1. Western blot analysis of PAF1 using anti-PAF1 antibody.)
Rabbit PAF1/PD2 Polyclonal Antibody | anti-PAF1 antibody
Anti-PAF1/PD2 Antibody
Gene Names
PAF1; PD2; F23149_1
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Synonyms
PAF1/PD2, Antibody; Anti-PAF1/PD2 Antibody; RNA polymerase II-associated factor 1 homolog; hPAF1; Pancreatic differentiation protein 2; PAF1; PD2; anti-PAF1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
No cross reactivity with other proteins.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
485
Applicable Applications for anti-PAF1 antibody
ELISA (Direct ELISA), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot)
Immunogen
E Coli-derived human PAF1/PD2 recombinant protein (Position: N18-D355).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen store at -20 degree C for a longer time. Avoid repeated freezing and thawing.
WB (Western Blot)
(Figure 1. Western blot analysis of PAF1 using anti-PAF1 antibody.)
WB (Western Blot)
(Figure 1. Western blot analysis of PAF1 using anti-PAF1 antibody.)
IF (Immunofluorescence)
IF (Immunofluorescence)
Related Product Information for anti-PAF1 antibody
Description: Rabbit IgG polyclonal antibody for PAF1/PD2 detection. Tested with WB, ICC/IF, Direct ELISA in Human; Mouse; Rat.
Background: PAF1 encodes a subunit of the polymerase associated factor (PAF1) complex. It is mapped to 19q13.2. The PAF1 complex interacts with RNA polymerase II and plays a role in transcription elongation as well as histone modifications including ubiquitylation and methylation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Background: PAF1 encodes a subunit of the polymerase associated factor (PAF1) complex. It is mapped to 19q13.2. The PAF1 complex interacts with RNA polymerase II and plays a role in transcription elongation as well as histone modifications including ubiquitylation and methylation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
References
1. Chen, F. X., Xie, P., Collings, C. K., Cao, K., Aoi, Y., Marshall, S. A., Rendleman, E. J., Ugarenko, M., Ozark, P. A., Zhang, A., Shiekhattar, R., Smith, E. R., Zhang, M. Q., Shilatifard, A. PAF1 regulation of promoter-proximal pause release via enhancer activation. Science 357: 1294-1298, 2017.
2. Marazzi, I., Ho, J. S. Y., Kim, J., Manicassamy, B., Dewell, S., Albrecht, R. A., Seibert, C. W., Schaefer, U., Jeffrey, K. L., Prinjha, R. K., Lee, K., Garcia-Sastre, A., Roeder, R. G., Tarakhovsky, A. Suppression of the antiviral response by an influenza histone mimic. Nature 483: 428-433, 2012.
3. Moniaux, N., Nemos, C., Schmied, B. M., Chauhan, S. C., Deb, S., Morikane, K., Choudhury, A., VanLith, M., Sutherlin, M., Sikela, J. M., Hollingsworth, M. A., Batra, S. K. The human homologue of the RNA polymerase II-associated factor 1 (hPaf1), localized on the 19q13 amplicon, is associated with tumorigenesis. Oncogene 25: 3247-3257, 2006.
2. Marazzi, I., Ho, J. S. Y., Kim, J., Manicassamy, B., Dewell, S., Albrecht, R. A., Seibert, C. W., Schaefer, U., Jeffrey, K. L., Prinjha, R. K., Lee, K., Garcia-Sastre, A., Roeder, R. G., Tarakhovsky, A. Suppression of the antiviral response by an influenza histone mimic. Nature 483: 428-433, 2012.
3. Moniaux, N., Nemos, C., Schmied, B. M., Chauhan, S. C., Deb, S., Morikane, K., Choudhury, A., VanLith, M., Sutherlin, M., Sikela, J. M., Hollingsworth, M. A., Batra, S. K. The human homologue of the RNA polymerase II-associated factor 1 (hPaf1), localized on the 19q13 amplicon, is associated with tumorigenesis. Oncogene 25: 3247-3257, 2006.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
RNA polymerase II-associated factor 1 homolog isoform 2
NCBI Official Synonym Full Names
PAF1 homolog, Paf1/RNA polymerase II complex component
NCBI Official Symbol
PAF1
NCBI Official Synonym Symbols
PD2; F23149_1
NCBI Protein Information
RNA polymerase II-associated factor 1 homolog
UniProt Protein Name
RNA polymerase II-associated factor 1 homolog
UniProt Gene Name
PAF1
UniProt Synonym Gene Names
PD2; hPAF1
UniProt Entry Name
PAF1_HUMAN
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Product Notes
The PAF1 paf1 (Catalog #AAA125435) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PAF1/PD2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PAF1/PD2 can be used in a range of immunoassay formats including, but not limited to, ELISA (Direct ELISA), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the PAF1 paf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PAF1/PD2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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