WB (Western Blot)
(Detection of human PARC/H7-AP1 by western blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PARC/H7-AP1 antibody AAA210761 (lot AAA210761-3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human PARC/H7-AP1 Polyclonal Antibody | anti-PARC/H7-AP1 antibody
Rabbit anti-PARC/H7-AP1 Antibody, Affinity Purified
Gene Names
CUL9; PARC; H7AP1
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
PARC/H7-AP1, Antibody; Rabbit anti-PARC/H7-AP1 Antibody, Affinity Purified; parkin-like cytoplasmic p53 binding protein; UbcH7-associated protein 1; PARC; H7AP1; cullin-9; CUL-9; p53-associated parkin-like cytoplasmic protein; Cullin-9; cullin 9; anti-PARC/H7-AP1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-PARC/H7-AP1 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 900 and 950
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human PARC/H7-AP1 by western blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PARC/H7-AP1 antibody AAA210761 (lot AAA210761-3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human PARC/H7-AP1 by western blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PARC/H7-AP1 antibody AAA210761 (lot AAA210761-3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human PARC/H7-AP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PARC/H7-AP1 antibody AAA210761 (lot AAA210761-3) used for IP at 6 ug per reaction. PARC/H7-AP1 was also immunoprecipitated by rabbit anti-PARC/H7-AP1 antibody For blotting immunoprecipitated PARC/H7-AP1, AAA210761 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human PARC/H7-AP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PARC/H7-AP1 antibody AAA210761 (lot AAA210761-3) used for IP at 6 ug per reaction. PARC/H7-AP1 was also immunoprecipitated by rabbit anti-PARC/H7-AP1 antibody For blotting immunoprecipitated PARC/H7-AP1, AAA210761 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-PARC/H7-AP1 antibody
PARC is a member of the cullin family of proteins that act as scaffolds to bring together ubiquitin E3 ligases, E2 conjugating enzyme, and substrate proteins to mediate the ubiquitination of substrate proteins. PARC is highly homologous to and can form heterodimers with CUL7 (cullin 7). PARC/H7 interacts with p53 and was initially thought to have a role in the sequestration of p53 to the cytoplasm. Recent studies demonstrate that PARC may not influence p53 function or localization, but rather p53 may serve to regulate PARC/H7-AP1 function.
Product Categories/Family for anti-PARC/H7-AP1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
268,362 Da
NCBI Official Full Name
cullin-9
NCBI Official Synonym Full Names
cullin 9
NCBI Official Symbol
CUL9
NCBI Official Synonym Symbols
PARC; H7AP1
NCBI Protein Information
cullin-9; CUL-9; UbcH7-associated protein 1; parkin-like cytoplasmic p53 binding protein; p53-associated parkin-like cytoplasmic protein
UniProt Protein Name
Cullin-9
UniProt Gene Name
CUL9
UniProt Synonym Gene Names
H7AP1; KIAA0708; PARC; CUL-9
UniProt Entry Name
CUL9_HUMAN
Similar Products
Product Notes
The PARC/H7-AP1 cul9 (Catalog #AAA210761) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-PARC/H7-AP1 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PARC/H7-AP1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the PARC/H7-AP1 cul9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PARC/H7-AP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.