Rabbit PARK7 Polyclonal Antibody | anti-PARK7 antibody

PARK7 Antibody

Cat. Num. AAA325951

• Gene Names: PARK7; DJ1; DJ-1; GATD2; HEL-S-67p
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Peptide affinity purification
• Synonyms: PARK7, Antibody; PARK7 Antibody; CAP1; DJ-1; DJ1; DJ1 protein; Epididymis secretory sperm binding protein Li 67p; FLJ27376; FLJ34360; FLJ92274; HEL S 67p; Oncogene DJ1; OTTHUMP00000001348; OTTHUMP00000001349; OTTHUMP00000001350; OTTHUMP00000001351; PARK7; PARK7_HUMAN; Parkinson disease (autosomal recessive, early onset) 7; Parkinson disease protein 7; Parkinson protein 7; Protein DJ-1; SP22; anti-PARK7 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PARK7 antibody detects endogenous levels of total PARK7
• Purity/Purification: Peptide affinity purification
• Form/Format: Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 189

• Applicable Applications for anti-PARK7 antibody: ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: A synthesized peptide derived from human PARK7 corresponding to a region within C-terminal amino acids
• Preparation and Storage: Store at -20°C. Stable for 12 months from date of receipt.

WB (Western Blot)

(Western blot analysis of extracts from HepG2, using PARK7 Antibody.)

IHC (Immunohiostchemistry)

(AAA325951 at 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IF (Immunofluorescence)

(AAA325951 staining HuvEc cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Red), diluted at 1/600, was used as secondary antibody.)

Related Product Information for anti-PARK7 antibody

Description: Parkinson's disease (PD) is characterized by the presence of Lewy bodies (intracellular inclusions) and by the loss of dopaminergic neurons. Research studies have shown that mutations in alpha-synuclein, Parkin, and DJ-1 are linked to PD (1). alpha-synuclein is a major component of the aggregates found in Lewy bodies. Parkin is involved in protein degradation through the ubiquitin-proteasome pathway, and investigators have shown that mutations in Parkin cause early onset of PD (1). Loss-of-function mutations in DJ-1 cause early onset of PD, but DJ-1 is associated with multiple functions: it cooperates with Ras to increase cell transformation, it positively regulates transcription of the androgen receptor, and it may function as an indicator of oxidative stress (2-5).

NCBI and Uniprot Product Information

• NCBI GI #: 183227678
• NCBI GeneID: 11315
• NCBI Accession #: NP_001116849.1
• NCBI GenBank Nucleotide #: NM_001123377.1
• UniProt Accession #: Q99497; O14805; Q6DR95; Q7LFU2; B2R4Z1
• Molecular Weight: 20 kDa
• NCBI Official Full Name: protein/nucleic acid deglycase DJ-1
• NCBI Official Synonym Full Names: Parkinsonism associated deglycase
• NCBI Official Symbol: PARK7
• NCBI Official Synonym Symbols: DJ1; DJ-1; GATD2; HEL-S-67p
• NCBI Protein Information: protein/nucleic acid deglycase DJ-1
• UniProt Protein Name: Protein/nucleic acid deglycase DJ-1
• UniProt Gene Name: PARK7
• UniProt Synonym Gene Names: DJ-1